The test detects cat (Felis catus) DNA and dog (Canis lupus familiaris) DNA seperately. Each reaction contains an internal amplification control and an internal detection assay for animal DNA (IAAC).
For DNA-preparation of raw material the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of three fluorescence emissions at 522 nm, 553 nm and 670 nm at the same time. The real-time PCR assay can be used with established real-time PCR instruments. The technical validation of instruments was performed on Roche LightCycler® 480 II, Roche cobas z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96 and Agilent Mx3005P.
|Test format||100 reactions|
|LOD (Detection limit)||The SureFood® ANIMAL ID 3plex Cat/Dog+IAAC real-time PCR is developed for the detection of cat and dog DNA in a DNA mixture at a relative amount of 0.5 %. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.|