The test is developed for the relative estimation of the 35S CaMV DNA amount in only Corn containing products.
Therefore the kit contains two PCR systems, one specific for the 35S CaMV promoter sequence and the other one specific for Corn (the reference gene). The detection of 35S CaMV promoter is according to German Food Law § 64 LFGB.
Because different GMO Corn events contain a different number of 35S promoter copies, systematically deviations may appear.
The real-time PCR assay can be used with established real-time PCR instruments (Roche LightCycler®, Rotor-Gene Q, ABI PRISM, Eppendorf realplex, BioRad CFX96, Agilent MxSeries etc.).
|2×50 reactions, whereof 50 reactions are used for the quantification of the reference gene.
|LOD (Detection limit)
|The 35S Corn PCR has a limit of detection of ca. 5 DNA-copies. The assay limit of detection depends on sample matrix, processing grade, DNA-preparation and DNA-content.
The limit of quantitation depends on the concentration of the sample DNA used in the analysis. For example, if 50,000 target-sequence copies of Corn specific reference gene are present, the relative quantitation limit for 35S Corn DNA is 0.1 %.