The test detects the relative quantitative GT73 Canola DNA amount. Therefore the kit contains two PCR systems, one specific for the GT73 Canola (OECD unique identifier MON-ØØØ73-7) and the other one specific for Brassicacea family (reference system).
The event specific detection is according to the official method of the European Commission.
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Bio-Rad CFX96, Roche LightCycler® 480 II, Applied Biosystems 7500, Qiagen Rotor-Gene Q and R-Biopharm RIDA®CYCLER.
|Test format||2 x 50 reactions|
|LOD (Detection limit)||The GT73 Canola PCR has a limit of detection of ≤ 5 DNA-copies. The assay limit of detection depends on
sample matrix, processing grade, DNA-preparation and DNA-content.
The limit of quantitation depends on the concentration of the sample DNA used in the analysis. For
example, if 50,000 target-sequence copies of Brassicacea specific reference gene are present, the relative
quantitation limit for GT73 Canola DNA is 0.1 %.
|Cross reactivity||The reference PCR system is specific for the Brassicacea family. That means that quantification is only
possible for samples in which canola material is known to be the only Brassicacea type. Therefore the GT73 Canola quantification in a mustard sample is not possible.