This kit can be used for screening of genetically modified organisms (GMOs) in food, feed and seeds. The multiplex test detects the following DNA-sequences:
- Phosphinothricin-Acetyltransferase gene (BAR) from Streptomyces hygroscopius
- Antibiotics-resistance gene Neomycin-Phosphotransferase (nptII)
- Phosphinothricin-Acetyltransferase gene (PAT) from Streptomyces viridiochromogenes
- The transition from CTP2 (Chloroplast-Transpeptide-signal sequence from Arabidopsis thaliana) to herbicide tolerance-gene CP4 EPSPS (5-Enolpyruvylshikimat-3-Phosphat Syntheses gene from Agrobacterium tumefaciens strain CP4)
The kit is according to German Food Law § 64 for the detection of genetically modified DNA sequences especially according to technical specification BVL L-00.00-124.
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of four fluorescence emissions at 510 nm, 580 nm, 610 nm und 660 nm (FAM, VIC, ROX and Cy5) at the same time. The technical validation of instruments was performed on Roche LightCycler® 480 II*, Applied Biosystems 7500, Bio-Rad CFX96 Agilent Mx3005P.
|Test format||100 reactions|
|Sample preparation||For DNA-preparation of raw material the use of SureFood® PREP Basic (S1052) and for highly processed food and feed the use of SureFood® PREP Advanced (S1053) is recommended.|
|LOD (Detection limit)||The SureFood® GMO SCREEN 4plex BAR/NPTII/PAT/CTP2:CP4 EPSPS real time PCR has a limit of detection of ≤ 5 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.|