The VitaFast® Vitamin B6 (Pyridoxin) microtiter plate test is a microbiological method for the quantitative determination of total vitamin B6 (added and natural vitamin B6) in food, animal feed and in pharmaceutical products. The microbiological test system is in accordance with international norms.
Vitamin B6 is extracted from a homogenized sample and the extract is diluted. The diluted extract and standards are applied to the wells of a microtiter plate coated with Saccharomyces cerevisiae. The growth of the bacteria is dependent on the vitamin content of the sample, or standard. Saccharomyces cerevisiae belongs to the yeast bacteria, and has an absolute growth requirement for the vitamin B6. Thus, this organism is ideal for quantification of pyridoxin in food and dietary supplements. As a group, the yeast bacteria are good candidates for vitamin analysis due to their genetic stability (resistance to undergo mutation), simple metabolic requirements, ease of maintenance and general safety as a non-pathogenic organism.
- The test system is very robust and well suited for routine analysis.
- The VitaFast® test kits feature uniform procedures across the product line.
|Test format||Microtiter plate with 96 wells (12 strips with 8 removable wells each)|
|Sample preparation||– Liquid samples (added vitamin B6): enzymatic treatment, extraction, sterile filtration and dilution.
– Solid samples (added vitamin B6): sample homogenization, enzymatic treatment, extraction, centrifugation and dilution.
– Liquid and solid samples (added and native vitamin B6): sample homogenization, enzymatic treatment, extraction, centrifugation and dilution.
|Incubation time||44 – 48 h in the dark at 30 °C (86 °F)|
|LOD (Detection limit)||0.0002 mg / 100 g (ml)|
|LOQ (Limit of quantification)||0.002 mg / 100 g (ml)|
|Validated matrices||Multivitamin juice, fitness drink, fruit gums, candies, pills, capsules, vitamin mixes, cereals, baby food, bread, flour, meat, meat products, milk products, yeast, yeast products.|
|Detected analyte||Pyridoxin concentration|
|Evaluation||Microtiter plate photometer 610-630 nm (alternativ 540-550 nm)|