Macrolides
Antibiotic macrolides are used to treat infections caused by Gram-positive bacteria (e.g., Streptococcus pneumoniae) and limited Gram-negative bacteria (e.g., Bordetella pertussis, Haemophilus influenzae), and some respiratory tract and soft-tissue infections. Beta-hemolytic streptococci, pneumococci, staphylococci, and enterococci are usually susceptible to macrolides. Unlike penicillin, macrolides have been shown to be effective against Legionella pneumophila, mycoplasma, mycobacteria, some rickettsia, and chlamydia.
Macrolides are not to be used on non-ruminant herbivores, such as horses and rabbits. They rapidly produce a reaction causing fatal digestive disturbance.
The screening for tylosin and erythromycin is an important tool for control in the honey industry.
EuroProxima Erythromycin
Intended use:
A competitive enzyme immunoassay for screening and quantitative analysis of erythromycin in various matrices.
General information:
Erythromycin is an antibiotic with a broad-spectrum activity, which belongs to the group of macrolides. Maximum Residue Limits (MRLs) for erythromycin for all food producing species are established in Europe: for tissue at 200 ppb, milk at 40 ppb and eggs at 150 ppb. The screening for tylosin and erythromycin is an important tool for control in the honey industry. The use of antibiotics in apiculture is forbidden. Therefore, no MRLs have been set for honey. Nevertheless, Recommended Concentrations (RCs) have been published by the European Union Reference Laboratories. For erythromycin the RC is 20 ppb.
The Erythromycin ELISA is a competitive enzyme immunoassay based on antibodies directed against erythromycin.
| Art. No. | 5151ERY |
|---|---|
| Test format | Microtiter plate, 96 wells. |
| Sample preparation | For milk, honey, egg, shrimps, fish, liver and urine fast and efficient extraction methods are included in the kit manual. |
| Incubation time | 90 minutes |
| Detection limit | Milk 4 ppb Honey 10 ppb Egg 10 ppb Shrimps 10 ppb Fish 10 ppb Liver 10 ppb Urine 4 ppb |
| Cross reactivity | Erythromycin 100%, Erythromycin ethylsuccinate 12%, Tylosin 0%, Spiramycin 0% |
EuroProxima Tylosin
Intended use:
A competitive enzyme immunoassay for screening and quantitative analysis of tylosin in various matrices.
General information:
Tylosin is a macrolide antibiotic that is active against Gram-positive bacteria and mycoplasmas. It is used in pigs, cattle and poultry for the treatment of conditions caused by sensitive organisms. In beekeeping tylosin is used to prevent infection diseases like American foulbrood. Tylosin consists predominantly of tylosin A and varying amounts of tylosin B, tylosin C and tylosin D. In the past, tylosin has been used as a feed additive in animal feed to promote growth, but in 1998 it was banned in accordance with Council Regulation (EC) no. 2821/98.
The Tylosin ELISA is a competitive enzyme immunoassay based on antibodies directed against tylosin.
| Art. No. | 5151TYL |
|---|---|
| Test format | Microtiter plate, 96 wells. |
| Sample preparation | For milk, honey, egg, feed, tissue, serum and urine fast and efficient methods are included in the kit manual. |
| Incubation time | 75 minutes |
| Detection limit | Milk 2.5 ppb Honey 2.5 ppb Egg 2.5 ppb Feed 2.5 ppb Tissue 2.5 ppb Serum 2.5 ppb Urine 2.5 ppb |
| Cross reactivity | Tylosin A 100%, Tylosin B 36%, Chloramphenicol 0%, Fluoroquinolones 0%, Leucomalachite green 0%, Tetracyclines 0% |