Anabolic Steroids

 

Anabolic steroids, also known more properly as anabolic–androgenic steroids (AAS), are steroidal androgens that include natural androgens like testosterone as well as synthetic androgens that are structurally related and have similar effects to testosterone. They are anabolic and increase protein within cells, especially in skeletal muscles, and also have varying degrees of androgenic and virilizing effects, including induction of the development and maintenance of masculine secondary sexual characteristics such as the growth of facial and body hair. The word anabolic, referring to anabolism, comes from the Greek ἀναβολή anabole, “that which is thrown up, mound”. Androgens or AAS are one of three types of sex hormone agonists, the others being estrogens like estradiol and progestogens like progesterone.

Androgens

Androgens are natural or synthetic steroid hormones that stimulate or control the development and maintenance of male characteristics by binding to androgen receptors. The group of androgens includes many synthetic molecules in addition to natural testosterone. If animals are treated with androgens, drug residues may remain in the animal’s meat and milk. Our test systems can reliably detect androgen residues.

 

RIDASCREEN® Trenbolon

Art. No.: R2601

Intended use:

RIDASCREEN® Trenbolon is a competitive enzyme immunoassay for the quantitative analysis of trenbolone in urine, bile, meat, liver and feces.

General information:

Trenbolone acetate is a very efficient anabolic steroid with strong androgenic activity. Genotoxic activity has not yet been found in residues after proper use of trenbolone. However, trenbolone in increased concentrations induced cell transformation in a test using “syrian hamster embryonal cells”, and reacted weakly positive in the Ames-test.

Accessories:

  • RIDA® Trenbolon  Spiking Solution
  • RIDA® Trenbolon Assay Control (positive)
  • RIDA® Trenbolon Assay Control (negative)
Specifications
Art. No. R2601
Test format Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Sample preparation Hydrolysis, Chromatographic prepurification using RIDA® C18 column (Art. No.: R2002)
Incubation time 2 h 30 min

 

RIDA® Trenbolon Spiking Solution

Art. No.: R2699

Intended use:

RIDA® Trenbolon Spiking Solution can be used for the production of positive control samples suitable for the validation (e.g. determination of the recovery rate) of the RIDASCREEN® Trenbolon test (R2601).

 

Specifications
Art. No. R2699
Test format trenbolon in methanol
concentation: 50 ng/ml
volume: 1 ml
ready-to-use

 

Trenbolon Assay Control (negative)

Art. No.: R2608

General information:

5 ml of freeze-dried urine of calves, Trenbolon negative

Specifications
Art. No. R2608

 

RIDASCREEN® Methyltestosteron

Art. No.: R3611

Intended use:

RIDASCREEN® Methyltestosteron is a competitive enzyme immunoassay for the quantitative analysis of methyltestosterone in urine (porcine, bovine), meat (pork, beef), fish (salmon, pangasius, tilapia, trout, catfish) and liver (porcine, bovine).

General information:

The anabolic effects of methyltestosterone are comparable to those of testosterone, which is a natural male sexual hormone. Methyltestosterone is a synthetic steroid hormone and as testosterone illegally also illegally used as resource in cattle fattening.

Accessories:

  • RIDA® Methyltestosteron Spiking Solution
Specifications
Art. No. R3611
Test format Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Incubation time approx. 2 h 15 min

 

RIDA® Methyltestosteron Spiking Solution

Art. No.: R3699

Intended use:

RIDA® Methyltestosteron Spiking Solution can be used for the production of positive control samples suitable for the validation (e.g. determination of the recovery rate) of the RIDASCREEN® Methyltestosteron test (R3611).

 

Specifications
Art. No. R3699
Test format methyltestosterone in methanol
concentration: 100 ng/ml
volume: 1 ml
ready-to-use

 

RIDASCREEN® 19-Nortestosteron

Art. No.: R2801

Intended use:

RIDASCREEN® 19-Nortestosteron is a competitive enzyme immunoassay for the quantitative analysis of of 19-nortestosterone in urine.

General information:

The anabolic effects of 19-Nortestosterone are comparable to those of testosterone, but with less androgenic effects. In Germany, 19-Nortestosterone-decanoate and -laureate are permitted for veterinary use. However, the illegal use of 19-Nortestosterone as doping agents in high performance sports or horse sports are far more common. Some  years ago the increasing application of illegal cocktails as a fattening resource in calf fattening was reported. Those cocktails usually contained 19-Nortestosterone-ester.

Accessories:

  • RIDA® 19-Nortestosteron Spiking Solution
Specifications
Art. No. R2801
Test format Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Sample preparation hydrolysis and chromatographic purification with RIDA® C18 columns (Art. No.: R2002)
Incubation time 1 h 15 min
Detection limit 3 µg/l (ppb)
(the measuring range in consideration of the dilution factor is 100 – 6000 ng/lppt)(corresponding to the standard substance)
Cross reactivity 19-Nortestosterone 17-β (standard substance): 100 %
19-Nortestosterone 17-α: approx. 80 %
19-Norethisterone: approx. 74 %
19-Norandrostendione: approx.100 %
18-Methyl-19 NT-17 β: approx. 59 %
17-α-Ethyl-19 NT-17 β: approx. 40 %
15-α, 16-α-Methyl.-19 NT-17-β-acetate: approx. 71 %
Trenbolone: approx. 10 %
17- β-Estradiol: approx. 0.1
Zeranol: < 0.1 %
DES: < 0.1 %
MPA: < 0.1 %

 

RIDA® 19-Nortestosteron Spiking Solution

Art. No.: R2899

Intended use:

RIDA® 19-Nortestosteron Spiking Solution can be used for the production of positive control samples suitable for the validation (e.g. determination of the recovery rate) of the RIDASCREEN® 19-Nortestosteron test (R2801).

Specifications
Art. No. R2899
Test format 19-nortestosterone in methanol
concentration: 1 μg/ml
volume: 1 ml
ready-to-use

 

RIDASCREEN® Ethinylöstradiol

Art. No.: R2511

Intended use:

RIDASCREEN® Ethinylöstradiol is a competitive enzyme immunoassay for the quantitative analysis of ethinylestradiol residues in urine, meat and plasma.

General information:

Ethinylestradiol is a synthetic sexual hormone which is, like all other natural or synthetic steroid  hormones, prohibited for use as resource in fattening in the European Community since 1988.

Accessories:

  • RIDA® Ethinylöstradiol Spiking Solution
Specifications
Art. No. R2511
Test format Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Incubation time 2 h 30 min

 

RIDA® Ethinylöstradiol Spiking Solution

Art. No.: R2599

Intended use:

RIDA® Ethinylöstradiol Spiking Solution can be used for the production of positive control samples suitable for the validation (e.g. determination of the recovery rate) of the RIDASCREEN® Ethinylöstradiol test (R2501).

Specifications
Art. No. R2599
Test format ethinylestradiol in methanol
concentration: 20 ng/ml
volume: 1 ml
ready-to-use

 

EuroProxima Progesterone

Art. No.: 5081PROG

Intended use:

A microtiter plate based competitive enzyme immunoassay for screening and quantitative analysis of Progesterone in milk and serum samples.

General information:

The Progesterone ELISA is a competitive enzyme immunoassay for the quantitative analysis of progesterone in milk and serum samples. It’s measurement in milk serves as a valuable diagnostic tool in identifying ovarian conditions such as (silent) heat, lack of cyclists, cystic follicles, retained corpora lutea and irregular cycles.

The Progesterone ELISA is a competitive enzyme immunoassay based on antibodies directed against progesterone.

Specifications
Art. No. 5081PROG
Test format Microtiter plate, 96 wells.
Sample preparation For milk and serum fast and efficient methods are included in the kit manual.
Incubation time 90 minutes
Detection limit Milk 1 ppb
Serum 1 ppb
Cross reactivity Progesterone 100%, Hydroxyprogesterone < 0.1%, 17-β-Testosterone < 0.1%, Esterone < 0.1%, 17-β-Nortestosterone < 0.1%

 

EuroProxima Stanozolol

Art. No.: 5081STAN

Intended use:

A competitive enzyme immunoassay for screening and quantitative analysis of stanozolol in various matrices.

General information:

Stanozolol is an anabolic steroid derived from dihydrotestosterone. Use of this substance as growth promotor in livestock is banned in the European Union. It can be used as anabolic compound in meat production. The use as doping agent in horse racing is another illegal application of stanozolol.

The Stanozolol ELISA is a competitive enzyme immunoassay based on antibodies directed against stanozolol.

Specifications
Art. No. 5081STAN
Test format Microtiter plate, 96 wells.
Sample preparation For urine and faeces fast and efficient methods are included in the kit manual.
Incubation time 45 minutes
Detection limit Urine 1 CCβ
Faeces 1 CCβ
Cross reactivity Stanozolol 100%, 16-β-hydroxy stanozolol 100%,
4-β-hydroxy stanozolol 0.30%, 3’ hydroxy stanozolol < 0.01%, Methyltestosterone < 0.01%, Testosterone < 0.01%