Aflatoxin

General information on aflatoxin

Aflatoxin B1 appears nearly in all cases together with Aflatoxin B2, G1 and G2 and it is the analyte with the highest toxic significance. It is commonly found in corn, peanuts, brazil nuts, cotton seed and pistachios.

Aflatoxin M1 is produced as a metabolite of aflatoxin B1. It is secreted with the milk after feeding of aflatoxin B1 containing feed to lactating cows. As aflatoxin M1 is relatively stable towards the pasteurizing process, not only a comprehensive routine check of the raw materials to be processed is required, but also of the final products.

Aflatoxin testsystems from R-Biopharm

We offer different analytical platforms for the analysis of aflatoxins as well as standards and reference materials.

RIDA®QUICK Aflatoxin

Art. No.: R5204

Intended use:

RIDA®QUICK Aflatoxin is a semi-quantitative immunochromatographic test in strip format for the detection of aflatoxin in grain, soy flour, nuts, pistachios, coconut flour, sunflower seeds, figs, dates and cashew nuts.

General Information:

Visual evaluation

**Specifications**
Art. No.	R5204
Test Format	20 x test strips (one for each determination, separately packed)
Sample Preparation	homogenization and extraction
Incubation Time	approx. 4 – 16 min
Detection Limit	The RIDA®QUICK Aflatoxin test is capable of detecting aflatoxin contaminations of ≥ 4 μg/kg (ppb), 10 μg/kg (ppb) and 20 μg/kg (ppb) depending on incubation time and color intensity of the test band. A positive result indicates that the sample has an aflatoxin content of ≥ 4 μg/kg (ppb).
Specificity	The RIDA®QUICK Aflatoxin test reacts with aflatoxin in grain (corn, wheat, barley, rye, oats, rice, millet, canola), soy flour, nuts (peanut, hazelnut, almond, Brazil nut, Macadamia nut), pistachios, coconut flour, sunflower seeds, figs, dates and cashew nuts.

RIDA®QUICK Aflatoxin RQS

Art. No.: R5205

Intended use:

RIDA®QUICK Aflatoxin RQS is a quantitative immunochromatographic test in strip format for the determination of aflatoxin in corn. Evaluation is performed by RIDA®QUICK SCAN or RIDA® SMART APP.

**Specifications**
Art. No.	R5205
Test Format	20 x test strips (one for each determination, separately packed)
Sample Preparation	homogenization and extraction
Incubation Time	5 min
Detection Limit	approx. 4 ppb
Specificity	The RIDA®QUICK Aflatoxin RQS test detects aflatoxin in corn.

RIDA®QUICK Aflatoxin RQS ECO

Art. No.: R5206

Intended use:

RIDA®QUICK Aflatoxin RQS ECO is a quantitative immunochromatographic test in strip format for the determination of aflatoxin in corn (maize). Evaluation is performed by RIDA®QUICK SCAN or RIDA® SMART APP.

The test uses an aqueous extraction method.

Specifications
Art. No.	R5206
Test format	20 test strips

AFLACARD TOTAL

Art. No.: P38

Intended use:

A qualitative screening card for detection of aflatoxins B1, B2, G1 and G2 at various levels in food and feed commodities.

General information:

The kit is based on monoclonal antibody technology which has the advantage of being highly specific and sensitive while the test format is rapid and simple to perform. The screening procedure is intended to serve as an indicator of the presence of toxins at various screening levels according to international legislation.

The toxins are extracted from the sample, filtered and passed through a clean-up column before being diluted and applied to the card. The conjugate is applied to the membrane and unbound conjugate is then removed by washing. A colourless substrate is added and the card is incubated for five minutes. Finally a stop solution is applied to the membrane. A purple spot must appear at the control site to indicate that the test is valid. A purple spot at the sample site shows that the contamination is less than the cut off value of the card. No colour at the sample site indicates contamination at a higher level than the cut off of the card.

**Specifications**
Art. No.	P38
Test Format	10 cards containing 20 tests and controls.
Sample Preparation	The toxins are extracted from the sample, filtered and passed through a clean-up column before being diluted and applied to the card. The conjugate is applied to the membrane and unbound conjugate is then removed by washing. A colourless substrate is added and the card is incubated for five minutes. Finally, a stop solution is applied to the membrane. A purple spot must appear at the control site to indicate that the test is valid. A purple spot at the sample site shows that the contamination is less that the cut off value of the card. No colour at the sample site indicates contamination at a higher level than the cut off of the card.
Incubation Time	5 minutes.
Detection Limit	Varies depending on dilution used.
Shelf life	10 months at 2 – 8 °C.
Sensitivity	Complies with current EU Legislation and compares well to aflatoxin analysis by HPLC.
Rapid	The assay time is only 10 minutes.
Robust	Cards can be stored in the dark to provide a permanent record of results.
Easy to use	The results can be read visually so there is no need to buy expensive detection equipment.
Reliable	Uses a monoclonal antibody to detect total aflatoxins.
Adaptable	Methods available for a wide range of commodities.
Extraction solvents	Methanol.

AFLACARD B1

Art. No.: P27

Intended use:

A qualitative screening card for detection of aflatoxin B1 at various levels in food and feed commodities.

General information:

The toxin is extracted from the sample, filtered and passed through a clean-up column before being diluted and applied to the card. The conjugate is applied to the membrane and unbound conjugate is then removed by washing. A colourless substrate is added and the card is incubated for five minutes. Finally a stop solution is applied to the membrane. A purple spot must appear at the control site to indicate that the test is valid. A purple spot at the sample site shows that the contamination is less that the cut off value of the card. No colour at the sample site indicates contamination at a higher level than the cut off of the card.

**Specifications**
Art. No.	P27
Test Format	10 cards containing 20 tests and controls.
Sample Preparation	The toxin is extracted from the sample, filtered and passed through a clean-up column before being diluted and applied to the card. The conjugate is applied to the membrane and unbound conjugate is then removed by washing. A colourless substrate is added and the card is incubated for five minutes. Finally a stop solution is applied to the membrane. A purple spot must appear at the control site to indicate that the test is valid. A purple spot at the sample site shows that the contamination is less that the cut off value of the card. No colour at the sample site indicates contamination at a higher level than the cut off of the card.
Incubation Time	5 minutes.
Detection Limit	Varies depending on dilution used.
Shelf life	10 months at 2 – 8 °C.
Sensitivity	Complies with current EU Legislation and compares well to aflatoxin B1 analysis by HPLC.
Rapid	The assay time is only 10 minutes.
Robust	Cards can be stored in the dark to provide a permanent record of results.
Easy to use	The results can be read visually so there is no need to buy expensive detection equipment.
Reliable	Uses a monoclonal antibody to detect aflatoxin B1.
Adaptable	Methods available for a variety of commodities.
Extraction solvents	Methanol.

RIDASCREEN® Aflatoxin M1

Art. No.: R1121

Intended use:

RIDASCREEN® Aflatoxin M1 is a competitive enzyme immunoassay for the quantitative analysis of aflatoxin M1 in milk, milk powder and cheese.

General Information:

Aflatoxins are carcinogenic, highly toxic metabolites of the mold fungus varieties Aspergillus flavus and Aspergillus parasiticus. Aflatoxin M1 is produced as a metabolite of aflatoxin B1. It is secreted with the milk after feeding of aflatoxin B1 containing feed to lactating cows. As aflatoxin M1 is relatively stable towards the pasteurizing process, not only a comprehensive routine check of the raw materials to be processed is required, but also of the final products.
Since the first of January 1999 EU-wide uniform residue limits for aflatoxins exist. For aflatoxin M1 the limit has been fixed at 0.05 μg/l (50 ppt).

**Specifications**
Art. No.	R1121
Test Format	Microtiter plate with 96 wells (12 strips with 8 wells each)
Standard Range	0 ppt (zero standard), 5 ppt, 10 ppt, 20 ppt, 40 ppt, 80 ppt
Sample Preparation	milk: degreasing milk powder: reconstitution and degreasing cheese: extraction and degreasing
Incubation Time	1 h 15 min
Detection Limit	milk: 5 ppt milk powder (referring to reconstituted milk): 5 ppt milk powder (referring to g-weight): 50 ppt cheese: 50 ppt(corresponding to the standard substance)
Cross Reactivity	The specificity of the RIDASCREEN® Aflatoxin M1 was established by analyzing the cross-reactivity to corresponding mycotoxins in buffer system.Aflatoxin M1: 100 % Aflatoxin M2: < 10 %

RIDASCREEN®FAST Aflatoxin M1

Art. No.: R5812

Intended use:

RIDASCREEN®FAST Aflatoxin M1 is a competitive enzyme immunoassay for the quantitative analysis of aflatoxin M1 in milk and milk powder.

General Information:

Aflatoxins are secondary metabolites of the fungi species Aspergillus flavus, parasiticus and nomius, which are carcinogenic and highly toxic.
Aflatoxin M1 is produced as a metabolite of aflatoxin B1. It is secreted with the milk after the feeding of aflatoxin B1 content feedstuffs to lactating cows.
As aflatoxin M1 is relatively stable towards the pasteurizing process, not only a comprehensive routine check of the raw materials to be processed is required, but also of the final products.
Since the first of January 1999 EU-wide uniform residue limits for aflatoxins exist. For aflatoxin M1 the limit has been fixed at 0.05 μg/l (50 ppt).

**Specifications**
Art. No.	R5812
Test Format	Microtiter plate with 48 wells (6 strips with 8 removable wells each)
Standard Range	0 ppt (zero standard), 125 ppt, 250 ppt, 500 ppt, 1000 ppt, 2000 ppt
Sample Preparation	centrifugation
Incubation Time	15 min
Detection Limit	milk: < 125 ppt milk powder (referring to reconstituted milk): < 125 ppt(corresponding to the standard substance)

RIDASCREEN® Aflatoxin B1 30/15

Art. No.: R1211

Intended use:

RIDASCREEN® Aflatoxin B1 30/15 is a competitive enzyme immunoassay for the quantitative analysis of aflatoxin B1 in cereals and feed.

General Information:

Aflatoxins are secondary metabolites of the fungi species Aspergillus flavus, parasiticus and nomius. These fungi occur in humid tropical areas and the contamination of vegetable food takes place in the cultivable countries. Aflatoxins belong to the strongest natural occurring carcinogenic substances. Aflatoxin B1 appears nearly in all cases together with Aflatoxin B2, G1 and G2 and it is the analyte with the highest toxic significance. It is commonly found in corn, peanuts, brazil nuts, cotton seed and pistachios.
Depending on the toxicity of these mycotoxins in the countries of the EU equal limits are valid for aflatoxins, 2 ppb for aflatoxin B1 and 4 ppb for all aflatoxins in total.

**Specifications**
Art. No.	R1211
Test Format	Microtiter plate (12 strips with 8 removable wells each)
Standard Range	0 ppb (zero standard), 1 ppb, 5 ppb, 10 ppb, 20 ppb, 50 ppb
Sample Preparation	grinding, extraction, filtration and dilution
Incubation Time	45 min
Detection Limit	1 ppb
Cross Reactivity	The specificity of the RIDASCREEN® Aflatoxin B1 30/15 test was determined by analyzing the crossreactivities to corresponding substances.Aflatoxin B1: 100 % Aflatoxin G1: approx. 29 % Aflatoxin B2: approx. 13 % Aflatoxin G2: approx. 3.2 % #Aflatoxin M1: approx. 1.5 %

RIDASCREEN® Aflatoxin Total

Art. No.: R4701

Intended use:

RIDASCREEN® Aflatoxin Total is a competitive enzyme immunoassay for the quantitative analysis of aflatoxin residues in cereals and feed.

General Information:

Aflatoxins are secondary metabolites of the fungi species Aspergillus flavus, parasiticus und nomius. These fungi occur in humid tropical areas and the contamination of vegetable food takes place in the cultivable countries. Aflatoxins belong to the strongest natural occurring cancerogenic substances.
Aflatoxin B1 which is mostly found together with the aflatoxins B2, G1 and G2 is the one with the highest toxic importance. It is found above all in corn, peanuts, brazil nuts, cotton seed and pistachios.
Depending on the toxicity of these mycotoxins in the countries of the EU equal limits are valid for aflatoxins, 2 ppb for aflatoxin B1 and 4 ppb for all aflatoxins in total.

**Specifications**
Art. No.	R4701
Test Format	Microtiter plate with 96 wells (12 strips with 8 wells each)
Standard Range	0 ppb (zero standard), 0.05 ppb, 0.15 ppb, 0.45 ppb,1.35 ppb, 4.05 ppb
Sample Preparation	extraction, filtration and dilution
Incubation Time	45 min
Detection Limit	approx. 1.75 ppb(corresponding to the standard substance)
Cross Reactivity	The specificity of the RIDASCREEN® Aflatoxin Total test was established by analyzing the cross-reactivity to corresponding mycotoxins in buffer system.Aflatoxin B1: 100 % Aflatoxin B2: approx. 48 % Aflatoxin G1: approx. 75% Aflatoxin G2: approx. 18 %

RIDASCREEN®FAST Aflatoxin ECO

FGIS/GIPSA 2017-098

Art. No.: R5201

Intended use:

RIDASCREEN®FAST Aflatoxin ECO is a competitive enzyme immunoassay for the quantitative analysis of aflatoxin in corn.

Federal Grain Inspection Services / CERTIFICATE NO. FGIS/GIPSA 2017-098

General Information:

Aflatoxins are carcinogenic, highly toxic metabolites of the mould fungi Aspergillus flavus and Aspergillus parasiticus.
Aflatoxin B1, generally present together with aflatoxin B2, G1 und G2, is occurring mainly in cereals, corn, cotton-seed and some nuts.

**Specifications**
Art. No.	R5201
Test Format	Microtiter plate with 48 wells (6 strips with 8 removable wells each)
Sample Preparation	extraction, filtration, dilution
Incubation Time	8 min
Measurement Range	5 – 300 μg/kg (ppb)

RIDASCREEN®FAST Aflatoxin

Art. No.: R5202

Intended use:

RIDASCREEN®FAST Aflatoxin is a competitive enzyme immunoassay for the quantitative analysis of aflatoxin in cereals and feed.

General Information:

**Specifications**
Art. No.	R5202
Test Format	Microtiter plate with 48 wells (6 strips with 8 removable wells each)
Standard Range	0 ppb (zero standard), 1.7 ppb, 5 ppb, 15 ppb, 45 ppb
Sample Preparation	extraction, filtration, dilution
Incubation Time	15 min
Detection Limit	< 1.7 μg/kg (ppb)(corresponding to the standard substance)

RIDASCREEN®FAST Aflatoxin SC

Art. No.: R9002

Intended use:

RIDASCREEN®FAST Aflatoxin SC is a competitive enzyme immunoassay for the quantitative analysis of aflatoxin in cereals and feed.

Federal Grain Inspection Services / CERTIFICATE NO. FGIS 2004 – 101

General Information:

**Specifications**
Art. No.	R9002
Test Format	Microtiter plate with 48 wells (6 strips with 8 removable wells each)
Standard Range	Only standard 1 (zero standard) is included in the test kit. For calculation of results with the standard curve the measured absorbance for standard 1 (0 ppb) as well as the B/B0 values for standards 2 – 7 (2, 4, 10, 20, 50, 100 ppb) provided with the QS-certificate are needed.
Sample Preparation	extraction, filtration, dilution
Incubation Time	15 min
Detection Limit	approx. 2 µg/kg (ppb)

RIDA® Aflatoxin column

Art. No.: R5001 / R5002

Intended use:

RIDA® Aflatoxin columns are immunoaffinity columns for sample clean up prior to analysis of aflatoxins B1, B2, G1, G2 in food and feed. The columns are particularly suited for the clean up of difficult samples such as nuts, herbs, spices and tea leaves.

The RIDA® Aflatoxin columns are usable in combination with enzyme immunoassay RIDASCREEN® Aflatoxin Total (Art. No. R4701) for the quantitative determination of aflatoxins B1, B2, G1, G2.

General Information:

The RIDA® Aflatoxin columns are usable in combination with enzyme immunoassays (RIDASCREEN® Aflatoxin Total, R4701 and Aflatoxin M1 30/15, R1111) for the quantitative determination of aflatoxins B1, B2, G1, G2 und M1.
The sample preparation with the immunoaffinity columns simplifies and enhances the sample clean up procedure. Pure extracts are obtained, which can be analyzed by different analytical methods.

**Specifications**
Art. No.	R5001 (10) / R5002 (50)
Test Format	R5001: 10 immunoaffinity columns R5002: 50 immunoaffinity columns
Detection Limit	depending on sample volume applied and on detection method (e. g. for herbs, spices and tea leaves) analysed by RIDASCREEN® Aflatoxin Total test approx. 250 ng/kg (ppt)(corresponding to the standard substance)

AFLAPREP® M

Art. No.: RBRDP04 /RBRP04

Intended use:

Immunoaffinity columns for use in conjunction with an HPLC or LC-MS/MS for detection of aflatoxin M1 in milk and dairy products.

General information:

The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits. The columns contain a gel suspension of monoclonal antibody specific to the toxin of interest. The sample is filtered and passed slowly through the immunoaffinity column. Any toxin which is present in the sample is retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxin is then released from the column following elution with solvent. The eluate is collected prior to analysis by HPLC or LC-MS/MS.

Specifications
Art. No.	DP04 / P04
Test format	10 narrow format immunoaffinity columns (DP04) 25 narrow format immunoaffinity columns (P04)
Detection limit	LOD: 0.05 ng/ml
Sample Preparation	The columns contain a gel suspension of monoclonal antibody specific to the toxin of interest. The sample is filtered and passed through the immunoaffinity column. Any toxin which is present in the sample is retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxin is then released by the antibody following elution with solvent. The eluate is collected prior to analysis by HPLC or LC-MS/MS.
Cross Reactivity	Aflatoxin M2
Shelf life	18 months at 2 – 8 °C (or 12 months at 21 – 25 °C).
Sensitivity	Low % CV, excellent recoveries, capacity and limit of detection exceeds EU Legislation and CEN / AOAC requirements.
Approvals	International Dairy Federation approval. The columns have also been assessed in a number of European and CEN collaborative trials.
Rapid	Clean up in 20 minutes prior to detection by HPLC or LC-MS/MS.
Robust	Can be stored at room temperature.
Reliable	Uses a monoclonal antibody to selectivly isolate aflatoxins M1 and M2.
Adaptable	Used for analysis of milk, milk powder & dairy products.
Extraction solvents	Methanol and Acetonitrile.
Approvals	International Dairy Federation approval. The columns have also been assessed in a number of European and CEN collaborative trials.

AFLAPREP® M WIDE

Art. No.: P124 / P124B

Intended use:

Immunoaffinity columns for use in conjunction with an HPLC or LC-MS/MS for detection of aflatoxin M1 in milk and dairy products.

General information:

Comment:

AFLAPREP® M WIDE is the highest capacity column on the market.

Specifications
Art. No.	P124 / P124B
Test format	10 wide format immunoaffinity columns (P124) 50 wide format immunoaffinity columns (P124B)
Detection limit	LOD: 0.05 ng/ml

AFLAPREP®

Art. No.: RBRDP07/RBRP07

Intended use:

Immunoaffinity columns for use in conjunction with an HPLC or LC-MS/MS for detection of aflatoxins B1, B2, G1 and G2 in a wide range of commodities.

General information:

The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits. The columns contain a gel suspension of monoclonal antibody specific to the toxins of interest. Following extraction of the toxins the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxins are then released from the column following elution with solvent. The eluate is collected prior to analysis by HPLC or LC-MS/MS. Aflatoxins are required to be derivatised when analysed by HPLC.

**Specifications**
Art. No.	RBRDP07/RBRP07
Test Format	10 narrow format immunoaffinity columns (DP07) 50 narrow format immunoaffinity columns (P07).
Sample Preparation	The columns contain a gel suspension of monoclonal antibody specific to the toxins of interest. Following extraction of the toxins the sample extract is filtered, diluted and passed through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxins are then released by the antibody following elution with solvent. The eluate is collected and derivatised prior to analysis by HPLCor LC-MS/MS.
Detection Limit	LOD: B1 – 0.031 ng/ml B2 – 0.015 ng/ml G1 – 0.015 ng/ml G2 – 0.007 ng/ml
Shelf life	18 months at 2 – 8 °C (or 12 months at 21 – 25 °C).
Sensitivity	Low % CV, excellent recoveries, capacity and limit of detection exceeds EU Legislation and CEN / AOAC requirements.
Approvals	Used in CEN collaborative trials. Methods using these columns have been approved by the AOAC.
Rapid	Clean up in 20 minutes prior to detection by HPLC or or LC-MS/MS.
Robust	Can be kept at room temperature.
Flexible	Can be used with different extraction solvents for optimum recovery.
Reliable	Uses a monoclonal antibody to selectively isolate and concentrate aflatoxins B1, B2, G1 and G2.
Adaptable	The columns are suitable for testing a wide range of commodities.
Extraction solvents	Methanol or Acetonitrile.

EASI-EXTRACT® AFLATOXIN

Art. No.: RBRP71 / RBRP70N

Intended use:

Immunoaffinity columns for use in conjunction with an HPLC or LC-MS/MS for detection of aflatoxins B1, B2, G1, G2, M1 and M2 in a wide range of commodities.

General information:

The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits. The columns contain a gel suspension of monoclonal antibody specific to the toxins of interest. Following extraction of the toxins the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxins are then released from the column following elution with solvent. The eluate is collected prior to analysis by HPLC or LC-MS/MS. Aflatoxins are required to be derivatised when analysed by HPLC.

**Specifications**
Art. No.	RBRP71 / RBRP70N
Test Format	10 wide format immunoaffinity columns (RP71). 50 wide format immunoaffinity columns (RP70N).
Sample Preparation	The columns contain a gel suspension of monoclonal antibody specific to the toxins of interest. Following extraction of the toxins the sample extract is filtered, diluted and passed through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxins are then released by the antibody following elution with solvent. The eluate is collected and derivatised prior to analysis by HPLC.
Detection Limit	LOD: B1 – 0.031 ng/ml B2 – 0.015 ng/ml G1 – 0.015 ng/ml G2 – 0.007 ng/ml M1 – 0.0625 ng/ml
Cross Reactivity	M1 and M2.
Shelf life	18 months at 2 – 8 °C (or 12 months at 21 – 25 °C).
Sensitivity	Low % CV, excellent recoveries, capacity and limit of detection exceeds EU Legislation and CEN / AOAC requirements.
Approvals	The columns have been successfully used in CEN collaborative trials and methods using these columns have been approved by the AOAC.
Rapid	Clean up in 20 minutes prior to detection by HPLC or TLC.
Robust	Can be stored at room temperature.
Flexible	Can be used with different extraction solvents for optimum recovery.
Reliable	Uses a monoclonal antibody to selectively isolate and concentrate aflatoxins B1, B2, G1, G2, M1 and M2.
Adaptable	The columns are suitable for testing a wide range of commodities.
Extraction solvents	Methanol or Acetonitrile.

AFLAOCHRA PREP®

Art. No.: P89 / P89B

Intended use:

Multi-mycotoxin immunoaffinity columns for simultaneous detection of aflatoxins B1, B2, G1, G2 and ochratoxin A in a wide range of commodities in conjunction with HPLC or LC-MS/MS.

General information:

The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits. The columns contain a gel suspension of monoclonal antibodies specific to the toxins of interest. Following extraction of the toxins the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxins are then released from the column following elution with solvent. The eluate is collected and injected prior to analysis by HPLC or LC-MS/MS. Aflatoxins are required to be derivatised when analysed by HPLC.

**Specifications**
Art. No.	P89 / P89B
Test Format	10 narrow format immunoaffinity columns (P89). 50 narrow format immunoaffinity columns (P89B).
Sample Preparation	The columns contain a gel suspension of monoclonal antibody specific to the toxins of interest. Following extraction of the toxins the sample extract is filtered, diluted and passed through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxins are released from the antibody following elution with solvent. The eluate is collected and injected prior to analysis by HPLC or LC-MS/MS.
Detection Limit	LOD: B1 – 0.031 ng/ml B2 – 0.015 ng/ml G1 – 0.015 ng/ml G2 – 0.007 ng/ml OTA – 0.05 ng/ml
Cross Reactivity	Ochratoxin B (OTB)
Shelf life	18 months at 2 – 8 °C (or 12 months at 21 – 25 °C).
Sensitivity	Low % CV, excellent recoveries, capacity and limit of detection exceeds EU Legislation and CEN / AOAC requirements.
Rapid	Only one extraction and a single column clean up required taking as little as 20 minutes prior to detection by HPLC or LC-MS/MS. Simultaneous detection of total aflatoxins and ochratoxin A in one HPLC or LC-MS/MS run.
Robust	Can be stored at room temperature.
Reliable	Uses monoclonal antibodies to selectively isolate and concentrate aflatoxins B1, B2, G1, G2 and ochratoxin A.
Adaptable	The columns are suitable for testing a wide range of commodities.
Extraction solvents	Methanol.
Additional Information	Cost Effective: No need for two separate analyses, saving time and money.

AO ZON PREP®

Art. No.: P112 / P112B

Intended use:

Multi-mycotoxin immunoaffinity columns for simultaneous detection of aflatoxins B1, B2, G1, G2, ochratoxin A and zearalenone in a wide range of commodities in conjunction with HPLC or LC-MS/MS.

General information:

The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits. The columns contain a gel suspension of monoclonal antibody specific to the toxins of interest. Following extraction of the toxins the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxins are then released from the column following elution with solvent. The eluate is collected and injected prior to analysis by HPLC or LC-MS/MS. Aflatoxins are required to be derivatised when analysed by HPLC.

Specifications
Art. No.	P112 / P112B
Test format	10 wide format immunoaffinity columns (P112) 50 wide format immunoaffinity columns (P112B)

AOF MS-PREP®

Art. No.: P115 / P115B

Intended use:

Multi-mycotoxin immunoaffinity columns for simultaneous detection of aflatoxins B1, B2, G1, G2, ochratoxin A, fumonisins B1 and B2 in a wide range of commodities in conjunction with LC-MS/MS.

General information:

The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. The columns contain a gel suspension of monoclonal antibodies specific to the toxins of interest. Following extraction of the toxins the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibodies within the gel suspension. The column is washed to remove unbound material and the toxins are then released from the column following elution with solvent. The eluate is collected prior to analysis by LC-MS/MS.

Specifications
Art. No.	P115 / P115B
Test format	10 wide format immunoaffinity columns (P115) 50 wide format immunoaffinity columns (P115B)

11+Myco MS-PREP®

Art. No.: P128 / P128B

Intended use:

Multi-mycotoxin immunoaffinity columns for simultaneous detection of aflatoxins B1, B2, G1, G2, ochratoxin A, fumonisins B1 and B2, Deoxynivalenol, Zearalenone, T-2 and HT-2 in a wide range of commodities in conjunction with LC-MS/MS.

General information:

Specifications
Art. No.	P128 / P128B
Test format	10 wide format immunoaffinity columns (P128) 50 wide format immunoaffinity columns (P128B)

PuriTox Aflatoxin

Art. No.: P25

Intended use:

Solid phase clean-up columns for the purification of aflatoxins B1, B2, G1 and G2 from peanuts.

General information:

PuriTox Aflatoxin can be used for the clean-up of peanut samples by HPLC or LC-MS/MS. In addition, the columns can be used for the clean-up of pigmented samples before ELISA to reduce background interference. The clean-up columns can be used in conjunction with HPLC or LC-MS/MS for the analysis of peanut samples. The toxins are extracted from the sample, filtered and passed through the solid phase clean-up column.

Specifications
Art. No.	P25
Test Format	50 solid phase clean-up columns.
Sample Preparation	Extraction and filtration.
Shelf life	2 years at 21 – 25 °C.
Rapid	Clean-up in 10 minutes prior to detection by ELISA.
Robust	Can be stored at room temperature.
Reliable	An effective clean-up system producing cleaner filtrates for use with ELISA therefore increasing the accuracy.
Adaptable	The columns are suitable for testing a wide range of commodities.

PuriTox AflaZON

Art. No.: TC-M160

Intended use:

Solid phase clean-up columns for the purification of aflatoxins and zearalenone from cereals.

General information:

PuriTox AflaZON columns can be used for the simultaneous detection of aflatoxins and zearalenone by HPLC. The columns reduce background interference therefore producing better chromatography and improving accuracy of results. The clean-up columns can be used in conjunction with HPLC for the analysis of pigmented samples. The toxins are extracted from the sample, filtered and passed through the solid phase clean-up column.

**Specifications**
Art. No.	TC-M160
Test Format	25 solid phase syringe format clean-up columns
Sample Preparation	The toxins are extracted from the sample, filtered and passed through the solid phase clean-up column.The clean-up columns can be used in conjunction with HPLC or GC for the analysis of pigmented samples.
Detection Limit	Aflatoxin – 1 ng/ml Zearalenone – 50 ng/ml
Shelf life	3 years at 21 – 25 °C
Rapid	Clean-up in 30 minutes prior to detection by HPLC or GC.
Robust	Can be stored at room temperature.
Reliable	An effective clean-up system producing cleaner chromatography and increased accuracy.
Adaptable	The columns are suitable for testing a wide range of cereal samples.
Extraction solvents	Acetonitrile and Methanol.

PuriTox Total Myco-MS

Art. No.: TC-MT3000

Intended use:

Solid phase clean-up columns for the purification of 11 mycotoxins.

General information:

PuriTox Total Myco-MS can be used for the simultaneous detection of aflatoxins B1, B2, G1, G2, ochratoxin A, fumonisins B1 and B2, deoxynivalenol, zearalenone, T-2 and HT-2 by LC-MS/MS. The columns reduce background interference therefore producing better chromatography and improving accuracy of results. The clean-up columns can be used in conjunction with LC-MS/MS for the analysis of cereal samples. The toxins are extracted from the sample, filtered and passed through the solid phase clean-up column.

Specifications
Art. No.	TC-MT3000
Test format	25 solid phase syringe format clean-up columns

MycotoxinStandards
Aflatoxin
Citrinin
DON
Fumonisin
Multi-Toxins
Ochratoxin
Patulin
Reference Materials
T-2 Toxin / HT-2 toxin
Trichothecenes
Zearalenone
Accessories