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DON

 

General information on DON (Deoxynivalenol or Vomitoxin)

Deoxynivalenol (DON) belongs to the trichothecene group of mycotoxins and is formed by fungi of the genus Fusarium. Deoxynivalenol often occurs in plant products particularly in cereals. More as 150 trichothecenes are known and the mycotoxins deoxynivalenol, 3-acetyl- and 15-acetyl-deoxynivalenol are the toxins most frequently occurring in Europe and Northern America. The toxin concentrations found in wheat, corn or rice are often in the ppm range. Due to their high cytotoxic and immunosuppressive properties these toxins pose a risk to human and animal health.

DON testsystems from R-Biopharm

We offer different analytical platforms for the analysis of DON as well as standards and reference materials.

RIDA®QUICK DON

Art. No.: R5906

 

Intended use:

RIDA®QUICK DON is a semi-quantitative or quantitative immunochromatographic test in strip format for the detection of DON in grain (wheat, triticale and corn/maize).

Comment:

Semi-quantitative evaluation can be performed visually; quantitative evaluation is performed by RIDA®QUICK SCAN or RIDA® SMART APP.

General Information:

Deoxynivalenol belongs to the trichothecene group of mycotoxins and is formed by fungi of the genus Fusarium. Deoxynivalenol often occurs in plant products particularly in cereals. Of more than 100 known trichothecenes the mycotoxin deoxynivalenol is the most frequently occurring in Europe and Northern America.

Specifications
Art. No. R5904
Test Format 20 x test strips (one determination each / individually packed)
Sample Preparation homogenization and extraction
Incubation Time 5 min
Detection Limit according to the extraction: approx. 0.5 mg/kg (ppm) or approx. 1.25 mg/kg (ppm) A positive result indicates that the sample has a DON content of ≥ 1.25 mg/kg (ppm) or ≥ 0.5 mg/kg (ppm).
Specificity The RIDA®QUICK DON test reacts with DON in wheat, triticale and corn samples. The test has been validated for these matrices.

 

RIDA®QUICK DON RQS ECO

Art. No.: R5911

 

Intended use:

RIDA®QUICK DON is a quantitative immunochromatographic test in strip format for the detection of DON in grain (wheat, triticale and corn/maize), the  evaluation is performed by RIDA®QUICK SCAN or RIDA® SMART APP.

General Information:

Deoxynivalenol belongs to the trichothecene group of mycotoxins and is formed by fungi of the genus Fusarium. Deoxynivalenol often occurs in plant products particularly in cereals. Of more than 100 known trichothecenes the mycotoxin deoxynivalenol is the most frequently occurring in Europe and Northern America.

Specifications
Art. No. R5911
Test Format 20 x test strips (one determination each / individually packed)
Sample Preparation homogenization and extraction
Incubation Time 3 min
Detection Limit according to the extraction: approx. 0.25 mg/kg (ppm)
Specificity The RIDA®QUICK DON test reacts with DON in wheat, triticale and corn samples. The test has been validated for these matrices.

RIDASCREEN® DON

Art. No.: R5906

Intended use:

RIDASCREEN® DON is a competitive enzyme immunoassay for the quantitative analysis of DON in cereals, malt, feed, beer and wort.

General Information:

Deoxynivalenol belongs to the trichothecene group of mycotoxins and is formed by fungi of the genus Fusarium. Deoxynivalenol often occurs in plant products particularly in cereals. More as 150 trichothecenes are known and the mycotoxins deoxynivalenol, 3-acetyl- and 15-acetyl-deoxynivalenol are the toxins most frequently occurring in Europe and Northern America. The toxin concentrations found in wheat, corn or rice are often in the ppm range. Due to their high cytotoxic and immunosuppressive properties these toxins pose a risk to human and animal health.

Specifications
Art. No. R5906
Test Format Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Standard Range 0 ppb (zero standard), 3.7 ppb, 11.1 ppb, 33.3 ppb, 100 ppb
Sample Preparation cereals, malt, feed: extraction, filtration
beer: removing of CO2
wort: without sample preparation
Incubation Time 45 min
Detection Limit cereals, malt, feed: 18.5 ppb
beer: 3.7 ppb
wort: 3.7 ppb
Cross Reactivity The specificity of the RIDASCREEN® DON test was determined by analyzing the cross-reactivities to corresponding mycotoxins.Deoxynivalenol: 100 %
3-Acetyldeoxynivalenol: > 100 %
15-Acetyldeoxynivalenol: approx. 19 %
Nivalenol: approx. 4 %
Fusarenon X: < 1 %
T-2 Toxin: < 1 %

RIDASCREEN®FAST DON

Art. No.: R5901 / R5902

Intended use:

RIDASCREEN®FAST DON is a competitive enzyme immunoassay for the quantitative analysis of DON in cereals, malt and feed.

Wheat, barley, malted barley, oats and corn are approved by AOAC Research Institute according to the Performance Tested Method Program and FGIS (Federal Grain Inspection Services) program of the Grain Inspection, Packers and Stockyards Administration of the United States Department of Agriculture (USDA/GIPSA).

Certificates:

General Information:

Deoxynivalenol belongs to the trichothecene group of mycotoxins and is formed by fungi of the genus Fusarium. Deoxynivalenol often occurs in plant products particularly in cereals. Of the trichothecene mycotoxins deoxynivalenol, 3-acetyl- and 15-acetyl-deoxynivalenol are the toxins most frequently occurring in Europe and Northern America. The toxin concentrations found in wheat, corn or rice are often in the ppm range. Due to their high cytotoxic and immunosuppressive properties these toxins pose a risk to human and animal health.

Specifications
R5901
Test Format R5901: microtiter plate with 96 wells (12 strips with 8 removable wells each)
R5902: microtiter plate with 48 wells (6 strips with 8 removable wells each)
Standard Range 0 ppm (zero standard), 0.222 ppm, 0.666 ppm, 2 ppm, 6 ppm
Sample Preparation extraction and filtration
Incubation Time 8 min
Detection Limit limit of detection: < 0.2 mg/kg (ppm)
limit of quantification: 0.2 mg/kg (ppm) / oats: 0.36 mg/kg (ppm)

 

RIDASCREEN®FAST DON SC

Art. No.: R5905

Intended use:

RIDASCREEN®FAST DON SC is a competitive enzyme immunoassay for the quantitative determination of DON in cereals, malt and feed.

Comment:

Only standard 1 (zero standard) is included in the test kit. For calculation of results with the standard curve the measured absorbance for standard 1 (0 ppb) as well as the B/B0 values for standards 2 – 8 (0.074, 0.222, 0.666, 1.333, 2, 4, and 6 ppm) provided with the QS-certificate are needed.

Certificates:

  • FGIS/GIPSA – FGIS 2014 – 052

General Information:

Deoxynivalenol (DON) belongs to the trichothecene group of mycotoxins and is formed by fungi of the genus Fusarium. DON often occurs in plant products particularly in cereals. Of the trichothecene mycotoxins deoxynivalenol, 3-acetyl- and 15-acetyl-deoxynivalenol are the toxins most frequently occurring in Europe and Northern America. The toxin concentrations found in wheat, corn or rice are often in the ppm range. Due to their high cytotoxic and immunosuppressive properties these toxins pose a risk to human and animal health.

Specifications
Art. No. R5905
Test Format Microtiter plate with 48 wells (6 strips with 8 removable wells each)
Standard Range Only standard 1 (zero standard) is included in the test kit.
For calculation of results with the standard curve the measured absorbance for standard 1 (0 ppm) as well as the B/B0 values for standards 2 – 8 (0.074, 0.222, 0.666, 1.333, 2, 4, and 6 ppm) provided with the QS-certificate are needed.
Sample Preparation extraction and filtration
Incubation Time 8 min
Detection Limit 0.074 mg/kg (ppm)

 

 

DONPREP®

Art. No.: P50 / P50B

Intended use:

Immunoaffinity columns for use in conjunction with an HPLC or LC-MS/MS for detection of deoxynivalenol in a wide range of commodities.

General information:

The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits. The columns contain a gel suspension of monoclonal antibody specific to the toxin of interest. Following extraction of the toxin the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxin which is present in the sample is retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxin is then released from the column following elution with solvent. The eluate is collected, evaporated and reconstituted prior to analysis by HPLC or LC-MS/MS.

Specifications
Art. No. P50 / P50B
Test Format 10 wide format immunoaffinity columns (P50).
50 wide format immunoaffinity columns (P50B).
Sample Preparation The columns contain a gel suspension of monoclonal antibody specific to the toxin of interest. Following extraction of the toxin the sample extract is filtered, diluted and passed through the immunoaffinity column. Any toxin which is present in the sample is retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxin is then released by the antibody following elution with solvent. The eluate is collected, evaporated and reconstituted prior to analysis by HPLC.
Detection Limit 7.5 ng/ml.
Cross Reactivity 3 acetyl-DON.
Shelf life 18 months at 2 – 8 °C (or 12 months at 21 – 25 °C).
Sensitivity Low % CV, excellent recoveries, capacity and limit of detection exceeds EU Legislation.
Approvals External evaluations are available.
Rapid Clean up in 20 minutes prior to detection by HPLC.
Robust Can be stored at room temperature.
Reliable Uses a monoclonal antibody to selectively isolate and concentrate deoxynivalenol.
Adaptable The columns are suitable for testing a wide range of commodities.
Extraction solvents Methanol or Acetonitrile.
Additional Information Deoxynivalenol standards are available from R-Biopharm.
Email Link biotica@otenet.gr

DZT MS-PREP®

Art. No.: P73 / P73B

Intended use:

Multi-mycotoxin immunoaffinity columns for simultaneous detection of deoxynivalenol, zearalenone, T-2 and HT-2 in a wide range of commodities in conjunction with LC-MS/MS.

General information:

The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits. The columns contain a gel suspension of monoclonal antibodies specific to the toxins of interest. Following extraction of the toxins the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxins are then released from the column following elution with solvent. The eluate is collected prior to analysis by LC-MS/MS.

Specifications
Art. No. P73 / P73B
Test Format 10 narrow format immunoaffinity columns (P73).
50 narrow format immunoaffinity columns (P73B).
Sample Preparation The columns contain a gel suspension of monoclonal antibody specific to the toxins of interest. Following extraction of the toxins the sample extract is filtered, diluted and passed through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxins are then released by the antibody following elution with solvent. The eluate is collected prior to analysis by LC-MS/MS.
Detection Limit LOD: DON – 0.1 ng/ml
ZON – 0.05 ng/ml
T-2 – 0.05 ng/ml
HT-2 0.05 ng/ml
Cross Reactivity 3-acetyl DON, α-Zearalenol (α-ZOL), ß-Zearalenol (ß-ZOL), Zearalanone (ZAN), α-Zearalanol (α-ZAL) and Zeranol.
Shelf life 18 months at 2 – 8 °C (or 12 months at 21 – 25 °C).
Sensitivity Low % CV, excellent recoveries and limit of detection complies with EU Legislation.
Rapid Only one extraction and a single column clean-up required taking as little as 20 minutes prior to detection by LC-MS/MS. Simultaneous detection of deoxynivalenol, zearalenone, T-2 and HT-2 in one LC-MS/MS run.
Robust Can be stored at room temperature.
Reliable Uses monoclonal antibodies to selectively isolate and concentrate deoxynivalenol, zearalenone, T-2 and HT-2.
Adaptable The columns are suitable for testing a wide range of commodities.
Extraction solvents Methanol and Acetonitrile.
Additional Information Cost Effective: No need for three separate analyses, saving time and money.Improved Clean-Up: Reduces interference and therefore the need to use matrix matched standards.

 

 

 

11+Myco MS-PREP®

Art. No.: P128 / P128B

Intended use:

Multi-mycotoxin immunoaffinity columns for simultaneous detection of aflatoxins B1, B2, G1, G2, ochratoxin A, fumonisins B1 and B2, Deoxynivalenol, Zearalenone, T-2 and HT-2 in a wide range of commodities in conjunction with LC-MS/MS.

General information:

The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. The columns contain a gel suspension of monoclonal antibodies specific to the toxins of interest. Following extraction of the toxins the sample extract is filtered, diluted and passed slowly through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibodies within the gel suspension. The column is washed to remove unbound material and the toxins are then released from the column following elution with solvent. The eluate is collected prior to analysis by LC-MS/MS.

Specifications
Art. No. P128 / P128B
Test format 10 wide format immunoaffinity columns (P128)
50 wide format immunoaffinity columns (P128B)

PuriTox DON/NIV

Art. No.: TC-C210

Intended use:

Solid phase clean-up columns for the purification of trichothecenes from animal feed.

General Information:

PuriTox Trichothecene Plus can be used for the simultaneous detection of type A and B trichothecenes by GC. The columns reduce background interference therefore producing better chromatography and improving accuracy of results.

Specifications
Art. No. TC-C210
Test Format 25 solid phase gravity flow clean-up columns
Sample Preparation The toxins are extracted from the sample, filtered and passed through the solid phase clean-up column.The clean-up columns can be used in conjunction with GC for the analysis of pigmented samples.
Detection Limit NIV – 7.80 ng/ml, DON – 1.95 ng/ml
Cross Reactivity T-2, HT-2, diacetoxyscirpenol, neosolaniol, fusarenon X, 3-acetyl DON, 15-acetyl DON, DON, nivalenol
Shelf life 3 years.
Rapid Clean-up in 30 minutes prior to detection by GC
Robust Can be stored at room temperature
Reliable An effective clean-up system producing cleaner chromatography and increased accuracy.
Adaptable The columns are suitable for testing a wide range of cereal samples.
Extraction solvents Acetonitrile.

 

PuriTox Total Myco-MS

Art. No.: TC-MT3000

Intended use:

Solid phase clean-up columns for the purification of 11 mycotoxins.

General information:

PuriTox Total Myco-MS can be used for the simultaneous detection of aflatoxins B1, B2, G1, G2, ochratoxin A, fumonisins B1 and B2, deoxynivalenol, zearalenone, T-2 and HT-2 by LC-MS/MS. The columns reduce background interference therefore producing better chromatography and improving accuracy of results. The clean-up columns can be used in conjunction with LC-MS/MS for the analysis of cereal samples. The toxins are extracted from the sample, filtered and passed through the solid phase clean-up column.

Specifications
Art. No. TC-MT3000
Test format 25 solid phase syringe format clean-up columns

 

Mycotoxins

Mycotoxin Standards
Aflatoxin
Citrinin
DON
Fumonisin
Multi-Toxins
Ochratoxin
Patulin
Reference Materials
T-2 Toxin / HT-2 toxin
Trichothecenes
Zearalenone
Accessories