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T-2 Toxin / HT-2 toxin

General information on T2-toxin and HT-2 toxin

T-2 and HT-2 toxin belong to the trichothecene group of mycotoxins and are formed by fungi of the genus Fusarium. T-2 and HT-2 toxin are often found in agricultural commodities, although the incidence and the concentrations found show a broad regional variation. Due to their high cytotoxic and immunosuppressive mode of action T-2 / HT-2 toxins are a threat for human and animal health.

T-2 toxin / HT-2 toxin testsystems from R-Biopharm

We offer different analytical platforms for the analysis of T-2 toxin and for T-2 plus HT-2 toxin as well as standards andreference materials.

EASI-EXTRACT® T-2 & HT-2

Intended use:

Immunoaffinity columns for use in conjunction with an HPLC system for detection of T-2 and HT-2 in a wide range of commodities.

General Information:

The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits.

Specifications
Art. No. P43 / P43B
Test Format 10 wide format immunoaffinity columns (P43).
50 wide format immunoaffinity columns (P43B).
Sample Preparation The columns contain a gel suspension of monoclonal antibody specific to the toxins of interest.
Following extraction of the toxins the sample extract is filtered, diluted and passed through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxins are then released by the antibody following elution with solvent. The eluate is collected and derivatised prior to analysis by HPLC.
Detection Limit LOD: T-2: 0.05 ng/ml
HT-2: 0.05 ng/ml
Shelf life 18 months at 2 – 8 °C (or 12 months at 21 – 25 °C).
Sensitivity Low % CV, excellent recoveries and limit of detection complies with EU Legislation.
Rapid Clean up in 20 minutes prior to detection by HPLC.
Robust Can be stored at room temperature.
Reliable Uses a monoclonal antibody to selectively isolate and concentrate T-2 and HT-2.
Adaptable The columns are suitable for testing a wide range of commodities.
Extraction solvents Methanol or Acetonitrile.
Email Link biotica@otenet.gr

Trichothecene P Column

Intended use:

Solid phase clean-up columns for the purification of trichothecenes from cereals.

General Information:

TRICHOTHECENE P Columns can be used for the simultaneous detection of type A and B trichothecenes by GC. The columns reduce background interference therefore producing better chromatography and improving accuracy of results.

Specifications
Art. No. P51
Test Format 30 solid phase clean-up columns.
Sample Preparation The toxins are extracted from the sample, filtered and passed through the solid phase clean-up column.The clean-up columns can be used in conjunction with GC for the analysis of pigmented samples.
Detection Limit 50 ng/ml.
Cross Reactivity T-2, HT-2, diacetoxyscirpenol, neosolaniol, fusarenon X, 3-acetyl DON, 15-acetyl DON, DON, nivalenol.
Shelf life 2 years at 21 – 25 °C.
Rapid Clean-up in 30 minutes prior to detection by GC.
Robust Can be stored at room temperature.
Reliable An effective clean-up system producing cleaner chromatography and increased accuracy.
Adaptable The columns are suitable for testing a wide range of cereal samples.
Extraction solvents Acetonitrile.
Email Link biotica@otenet.gr

Trichothecene EP Column

Intended use:

Solid phase clean-up columns for the purification of trichothecenes from animal feed.

General Information:

PuriTox Trichothecene Plus can be used for the simultaneous detection of type A and B trichothecenes by GC. The columns reduce background interference therefore producing better chromatography and improving accuracy of results.

Specifications
Art. No. P53
Test Format 30 solid phase gravity flow clean-up columns.
Sample Preparation The toxins are extracted from the sample, filtered and passed through the solid phase clean-up column.The clean-up columns can be used in conjunction with GC for the analysis of pigmented samples.
Detection Limit 50 ng/ml.
Cross Reactivity T-2, HT-2, diacetoxyscirpenol, neosolaniol, fusarenon X, 3-acetyl DON, 15-acetyl DON, DON, nivalenol.
Shelf life 2 years at 21 – 25 °C.
Rapid Clean-up in 30 minutes prior to detection by GC.
Robust Can be stored at room temperature.
Reliable An effective clean-up system producing cleaner chromatography and increased accuracy.
Adaptable The columns are suitable for testing a wide range of animal feed samples.
Extraction solvents Acetonitrile.
Email Link biotica@otenet.gr

DZT MS-PREP®

Intended use:

Multi-mycotoxin immunoaffinity columns for simultaneous detection of deoxynivalenol, zearalenone, T-2 and HT-2 in a wide range of commodities in one LC-MS/MS run.

General Information:

The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Improved clean-up and concentration of toxins from complex food matrices results in reduced chromatography interference and lower detection limits.

Specifications
Art. No. P73 / P73B
Test Format 10 narrow format immunoaffinity columns (P73).
50 narrow format immunoaffinity columns (P73B).
Sample Preparation The columns contain a gel suspension of monoclonal antibody specific to the toxins of interest. Following extraction of the toxins the sample extract is filtered, diluted and passed through the immunoaffinity column. Any toxins which are present in the sample are retained by the antibody within the gel suspension. The column is washed to remove unbound material and the toxins are then released by the antibody following elution with solvent. The eluate is collected prior to analysis by LC-MS/MS.
Detection Limit LOD: DON – 0.1 ng/ml
ZON – 0.05 ng/ml
T-2 – 0.05 ng/ml
HT-2 0.05 ng/ml
Cross Reactivity 3-acetyl DON, α-Zearalenol (α-ZOL), ß-Zearalenol (ß-ZOL), Zearalanone (ZAN), α-Zearalanol (α-ZAL) and Zeranol.
Shelf life 18 months at 2 – 8 °C (or 12 months at 21 – 25 °C).
Sensitivity Low % CV, excellent recoveries and limit of detection complies with EU Legislation.
Rapid Only one extraction and a single column clean-up required taking as little as 20 minutes prior to detection by LC-MS/MS. Simultaneous detection of deoxynivalenol, zearalenone, T-2 and HT-2 in one LC-MS/MS run.
Robust Can be stored at room temperature.
Reliable Uses monoclonal antibodies to selectively isolate and concentrate deoxynivalenol, zearalenone, T-2 and HT-2.
Adaptable The columns are suitable for testing a wide range of commodities.
Extraction solvents Methanol and Acetonitrile.
Additional Information Cost Effective: No need for three separate analyses, saving time and money.Improved Clean-Up: Reduces interference and therefore the need to use matrix matched standards.
Email Link biotica@otenet.gr

PuriTox Trichothecene Plus

Intended use:

Solid phase clean-up columns for the purification of trichothecenes from animal feed.

General Information:

PuriTox Trichothecene Plus can be used for the simultaneous detection of type A and B trichothecenes by GC. The columns reduce background interference therefore producing better chromatography and improving accuracy of results.

Specifications
Art. No. TC-C210
Test Format 25 solid phase gravity flow clean-up columns
Sample Preparation The toxins are extracted from the sample, filtered and passed through the solid phase clean-up column.The clean-up columns can be used in conjunction with GC for the analysis of pigmented samples.
Detection Limit 50 ng/ml
Cross Reactivity T-2, HT-2, diacetoxyscirpenol, neosolaniol, fusarenon X, 3-acetyl DON, 15-acetyl DON, DON, nivalenol
Shelf life 3 years.
Rapid Clean-up in 30 minutes prior to detection by GC
Robust Can be stored at room temperature
Reliable An effective clean-up system producing cleaner chromatography and increased accuracy.
Adaptable The columns are suitable for testing a wide range of cereal samples.
Extraction solvents Acetonitrile.
Email Link biotica@otenet.gr

PuriTox Trichothecene

Intended use:

Solid phase clean-up columns for the purification of trichothecenes from cereals.

General Information:

PuriTox Trichothecene can be used for the simultaneous detection of type A and B trichothecenes by GC. The columns reduce background interference therefore producing better chromatography and improving accuracy of results.

Specifications
Art. No. TC-T220
Test Format 25 solid phase syringe format clean-up columns.
Sample Preparation The toxins are extracted from the sample, filtered and passed through the solid phase clean-up column.The clean-up columns can be used in conjunction with GC for the analysis of pigmented samples.
Detection Limit 50 ng/ml.
Cross Reactivity T-2, HT-2, diacetoxyscirpenol, neosolaniol, fusarenon X, 3-acetyl DON, 15-acetyl DON, DON, nivalenol
Shelf life 3 years at 21 – 25 °C.
Rapid Clean-up in 30 minutes prior to detection by GC.
Robust Can be stored at room temperature.
Reliable An effective clean-up system producing cleaner chromatography and increased accuracy.
Adaptable The columns are suitable for testing a wide range of cereal samples.
Extraction solvents Acetonitrile.
Email Link biotica@otenet.gr

RIDASCREEN® T-2 Toxin

Intended use:

RIDASCREEN® T-2 Toxin is a competitive enzyme immunoassay for the quantitative analysis of T-2 toxin in cereals and feed.

General Information:

T-2 toxin belongs to the trichothecene group of mycotoxins and is formed by fungi of the genus Fusarium. T-2 toxin is often found in agricultural commodities, although the incidence and the concentrations found show a broad regional variation. Due to its cytotoxic and immunosuppressive mode of action T-2 toxin is a threat for human and animal health.

Specifications
Art. No. R3801
Test Format Microtiter plate (12 strips with 8 removable wells each)
Standard Range 0 ppb (zero standard), 0.1 ppb, 0.2 ppb, 0.4 ppb, 0.8 ppb, 1.6 ppb
Sample Preparation extraction, filtration and dilution
Incubation Time 1 h 30 min
Detection Limit < 5 µg/kg (ppb)
Cross Reactivity The specificity of the RIDASCREEN® T-2 Toxin test was established by analyzing the cross-reactivity to corresponding mycotoxins.T-2 toxin: 100 %
Acetyl T-2 toxin: approx. 114 %
HT-2 toxin: approx. 7 %
Iso T-2 toxin: approx. 2 %

RIDASCREEN®FAST T-2 Toxin

Intended use:

RIDASCREEN®FAST T-2 Toxin is a competitive enzyme immunoassay for the quantitative analysis of T-2 toxin in cereals and feed.

The test was validated with the matrices corn, corn seedlings and mixed feed for chicken breeding and for fattened pigs.

General Information:

T-2 toxin belongs to the trichothecene group of mycotoxins and is produced by fungi of the genus Fusarium. T-2 toxin is often found in agricultural commodities, although the incidence and the concentrations found show a broad regional variation. Due to its cytotoxic and immunosuppressive activity, T-2 toxin is a threat for human and animal health.

Specifications
Art. No. R5302
Test Format Microtiter plate with 48 wells (6 strips with 8 removable wells each)
Standard Range 0 ppb (zero standard), 50 ppb, 100 ppb, 200 ppb, 400 ppb
Sample Preparation extraction, filtration and dilution
Incubation Time 15 min
Detection Limit limit of detection: < 20 µg/kg (ppb)
limit of quantification: 50 µg/kg (ppb)

Mycotoxins

Mycotoxin Standards
Aflatoxin
Citrinin
DON
Fumonisin
Multi-Toxins
Ochratoxin
Patulin
Reference Materials
T-2 Toxin / HT-2 toxin
Trichothecenes
Zearalenone
Accessories