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Trichothecenes

General information on trichothecene testing

The trichothecenes are a very large family of chemically related mycotoxins produced by molds like Fusarium, Trichoderma, Trichothecium and others. The trichothecenes are divided into two groups: macrocyclic and non-macrocyclic. T-2 is in the non-macrocyclic group, this group is also subdivided into the Type A and Type B trichothecenes. The Type A trichothecenes include T-2, HT-2, Neosolaniol and DAS as mycotoxin components, type B trichothecenes include DON, 3.acetyl- and 15 acetyl-DON, Nivalenol and Fusarenon-X.

Corn, wheat, barley, oats, rice, rye and other crops have been reported to contain trichothecenes. Natural occurrence has been reported in Asia, Africa, South America, Europe, and North America. Natural levels range from near zero to 10 ppm with a few exceptions showing levels of 15-40 ppm. The toxin production is greatest with increased humidity and temperatures of 6-24 °C.

The major toxic attribute of the trichothecenes is that they inhibit protein synthesis. It affects the actively dividing cells such as those lining the gastrointestinal tract, skin, lymphoid, and erythroid cells. The effects on animals include weight loss or poor weight gain, bloody diarrhea, dermal necrosis or beak lesions and decreased production (weight gain, eggs, milk, etc.). Type A trichothecenes are more toxic to poultry species than the Type B trichothecenes.

Trichothecene testsystems from R-Biopharm

Due to the relative large number of different structures common methods to analyze trichothecenes are GC or LC-MS methods. For this we offer different types of clean-up tools as well as standards and reference materials.

For the individual trichothecenes like DON and T-2 toxin we offer specific testsystems.

Trichothecene P Column

Intended use:

Solid phase clean-up columns for the purification of trichothecenes from cereals.

General Information:

TRICHOTHECENE P Columns can be used for the simultaneous detection of type A and B trichothecenes by GC. The columns reduce background interference therefore producing better chromatography and improving accuracy of results.

Specifications
Art. No. P51
Test Format 30 solid phase clean-up columns.
Sample Preparation The toxins are extracted from the sample, filtered and passed through the solid phase clean-up column.The clean-up columns can be used in conjunction with GC for the analysis of pigmented samples.
Detection Limit 50 ng/ml.
Cross Reactivity T-2, HT-2, diacetoxyscirpenol, neosolaniol, fusarenon X, 3-acetyl DON, 15-acetyl DON, DON, nivalenol.
Shelf life 2 years at 21 – 25 °C.
Rapid Clean-up in 30 minutes prior to detection by GC.
Robust Can be stored at room temperature.
Reliable An effective clean-up system producing cleaner chromatography and increased accuracy.
Adaptable The columns are suitable for testing a wide range of cereal samples.
Extraction solvents Acetonitrile.
Email Link biotica@otenet.gr

Trichothecene EP Column

Intended use:

Solid phase clean-up columns for the purification of trichothecenes from animal feed.

General Information:

PuriTox Trichothecene Plus can be used for the simultaneous detection of type A and B trichothecenes by GC. The columns reduce background interference therefore producing better chromatography and improving accuracy of results.

Specifications
Art. No. P53
Test Format 30 solid phase gravity flow clean-up columns.
Sample Preparation The toxins are extracted from the sample, filtered and passed through the solid phase clean-up column.The clean-up columns can be used in conjunction with GC for the analysis of pigmented samples.
Detection Limit 50 ng/ml.
Cross Reactivity T-2, HT-2, diacetoxyscirpenol, neosolaniol, fusarenon X, 3-acetyl DON, 15-acetyl DON, DON, nivalenol.
Shelf life 2 years at 21 – 25 °C.
Rapid Clean-up in 30 minutes prior to detection by GC.
Robust Can be stored at room temperature.
Reliable An effective clean-up system producing cleaner chromatography and increased accuracy.
Adaptable The columns are suitable for testing a wide range of animal feed samples.
Extraction solvents Acetonitrile.
Email Link biotica@otenet.gr

PuriTox Trichothecene Plus

Intended use:

Solid phase clean-up columns for the purification of trichothecenes from animal feed.

General Information:

PuriTox Trichothecene Plus can be used for the simultaneous detection of type A and B trichothecenes by GC. The columns reduce background interference therefore producing better chromatography and improving accuracy of results.

Specifications
Art. No. TC-C210
Test Format 25 solid phase gravity flow clean-up columns
Sample Preparation The toxins are extracted from the sample, filtered and passed through the solid phase clean-up column.The clean-up columns can be used in conjunction with GC for the analysis of pigmented samples.
Detection Limit 50 ng/ml
Cross Reactivity T-2, HT-2, diacetoxyscirpenol, neosolaniol, fusarenon X, 3-acetyl DON, 15-acetyl DON, DON, nivalenol
Shelf life 3 years.
Rapid Clean-up in 30 minutes prior to detection by GC
Robust Can be stored at room temperature
Reliable An effective clean-up system producing cleaner chromatography and increased accuracy.
Adaptable The columns are suitable for testing a wide range of cereal samples.
Extraction solvents Acetonitrile.
Email Link biotica@otenet.gr

PuriTox Trichothecene

Intended use:

Solid phase clean-up columns for the purification of trichothecenes from cereals.

General Information:

PuriTox Trichothecene can be used for the simultaneous detection of type A and B trichothecenes by GC. The columns reduce background interference therefore producing better chromatography and improving accuracy of results.

Specifications
Art. No. TC-T220
Test Format 25 solid phase syringe format clean-up columns.
Sample Preparation The toxins are extracted from the sample, filtered and passed through the solid phase clean-up column.The clean-up columns can be used in conjunction with GC for the analysis of pigmented samples.
Detection Limit 50 ng/ml.
Cross Reactivity T-2, HT-2, diacetoxyscirpenol, neosolaniol, fusarenon X, 3-acetyl DON, 15-acetyl DON, DON, nivalenol
Shelf life 3 years at 21 – 25 °C.
Rapid Clean-up in 30 minutes prior to detection by GC.
Robust Can be stored at room temperature.
Reliable An effective clean-up system producing cleaner chromatography and increased accuracy.
Adaptable The columns are suitable for testing a wide range of cereal samples.
Extraction solvents Acetonitrile.
Email Link biotica@otenet.gr

Mycotoxins

Mycotoxin Standards
Aflatoxin
Citrinin
DON
Fumonisin
Multi-Toxins
Ochratoxin
Patulin
Reference Materials
T-2 Toxin / HT-2 toxin
Trichothecenes
Zearalenone
Accessories