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DNA Preparation

SureFood® PREP Basic

Art. No.: S1052

Intended use:

This kit is intended to be used for the extraction of animal and plant DNA (deoxyribonucleic acid) from raw materials as well as slightly processed food and feed. It is also recommended for the extraction of animal DNA from highly processed food and feed.

Specifications
Art. No. S1052
Test Format 100 preparations
Sample Preparation DNA extraction and purification from food and feed-stuff of mainly animal origin.

 

SureFood® PREP Advanced

Art. No.: S1053

Intended use:

This kit is intended to be used for the extraction of animal and plant DNA (deoxyribonucleic acid). It can be used with two different protocols.

The DNA extraction protocol 1 is designed for the sensitive extraction of animal and plant DNA from food according to regulation (EU) 1169/2011. (Flowchart overview protocol 1).

The DNA extraction protocol 2 is designed for the extraction of plant DNA from highly processed food and feed. (Flowchart overview protocol 2). Furthermore, it can be used for samples that produce PCR inhibiting DNA extracts when extracted with protocol 1. Potentially occurring PCR inhibiting substances are efficiently removed in additional binding and purification steps.

Specifications
Art. No. S1053
Test format 50 preparations

 

SureFast® Animal+Plant Control 3plex

Art. No.: F4053

Intended use:

The SureFast® Animal+Plant Control 3plex real time PCR controls the DNA-preparation. In addition a differentiation between the DNA of vertebrates and plants is possible. The test contains an Internal Control DNA (ICD) as an internal control of sample preparation procedure and to determine possible PCR-inhibition.

General information:

The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of three fluorescence emissions at 522 nm, 553 nm and 670 nm (FAM, VIC/HEX and Cy5) at the same time. The technical validation of instruments was performed on Roche LightCycler® 480 II/Roche cobas z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, BMS MIC, Agilent Mx3005P and Agilent AriaDx.

Specifications
Art. No. F4053
Test format 100 reactions
Detection limit The SureFast® Animal+Plant Control 3plex real-time PCR has a limit of detection of < 500 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.

Animal Identification

DNA Preparation
Beef
Pork
Chicken / Turkey
Cat & Dog
Horse & Donkey
Fish
Rabbit