SureFood® PREP Basic
This kit is intended to be used for the extraction of animal and plant DNA (deoxyribonucleic acid) from raw materials as well as slightly processed food and feed. It is also recommended for the extraction of animal DNA from highly processed food and feed.
|Test Format||100 preparations|
|Sample Preparation||DNA extraction and purification from food and feed-stuff of mainly animal origin.|
SureFood® PREP Advanced
This kit is intended to be used for the extraction of animal and plant DNA (deoxyribonucleic acid). It can be used with two different protocols.
The DNA extraction protocol 1 is designed for the sensitive extraction of animal and plant DNA from food according to regulation (EU) 1169/2011. (Flowchart overview protocol 1).
The DNA extraction protocol 2 is designed for the extraction of plant DNA from highly processed food and feed. (Flowchart overview protocol 2). Furthermore, it can be used for samples that produce PCR inhibiting DNA extracts when extracted with protocol 1. Potentially occurring PCR inhibiting substances are efficiently removed in additional binding and purification steps.
|Test format||50 preparations|
SureFast® Animal+Plant Control 3plex
The SureFast® Animal+Plant Control 3plex real time PCR controls the DNA-preparation. In addition a differentiation between the DNA of vertebrates and plants is possible. The test contains an Internal Control DNA (ICD) as an internal control of sample preparation procedure and to determine possible PCR-inhibition.
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of three fluorescence emissions at 522 nm, 553 nm and 670 nm (FAM, VIC/HEX and Cy5) at the same time. The technical validation of instruments was performed on Roche LightCycler® 480 II/Roche cobas z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, BMS MIC, Agilent Mx3005P and Agilent AriaDx.
|Test format||100 reactions|
|Detection limit||The SureFast® Animal+Plant Control 3plex real-time PCR has a limit of detection of < 500 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.|