EuroProxima Azaperone-Azaperol is a competitive enzyme immunoassay for screening and quantitative analysis of azaperone/azaperol in various matrices.
Azaperone can be used as tranquilizer to prevent stress, f. e. during transportation of pigs. It effects the central nervous system and is thought to act by blocking dopamine receptors in the brain.
The major metabolite of azaperone is azaperol. For control purposes the marker residue is therefore the sum of azaperone and azaperol, as both compounds show pharmacological activity. In the EU azaperone is listed in Annex I of Council Regulation (EEC) No. 2377/90. Maximum residue limits (MRLs) of the marker residue have been set at 100 μg/kg (ppb) in porcine kidney and muscle tissue.
EuroProxima Azaperone-Azaperol ELISA is a competitive enzyme immunoassay based on antibodies against azaperone/azaperol.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each)|
|Sample preparation||For mentioned matrices fast and efficient extraction methods are included in the kit description.|
|Incubation time||1 h 30 min (20 – 25 °C)|
|LOD (Detection limit)||Tissue 3 ng/ml (ppb), liver 5 ng/ml, urine 0.2 ng/ml, kidney 10 ng/ml|
|Detection capability CCβ||Tissue 10 ng/ml (ppb), liver 10 ng/ml, urine 2 ng/ml|
|Validated matrices||Tissue, liver, urine and kidney.|
|Detected analyte||Azaperone/Azaperol in tissue, liver, urine or kidney.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|