A competitive enzyme immunoassay for screening and quantitative analysis of diethylstilbestrol (DES) in various matrices.
Diethylstilbestrol (DES) is banned in the European Union for use as growth promoter in livestock. Recommended concentration for screening tests have been set: 2 µg/kg (ppb) for liver and 1 µg/kg for urine and tissue.
- Low detection limit of 0.18 µg/kg for tissue and 0.16 µg/l for urine.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each)|
|Sample preparation||For tissue and urine fast and efficient methods are included in the kit manual under 8. Sample preparation.|
|Incubation time||1 h 30 min|
|LOD (Detection limit)||Tissue 0.18 µg/kg, urine 0.16 µg/l|
|Detection capability CCβ||Tissue 0.25 µg/kg, urine 0.20 µg/l|
|Validated matrices||Tissue and urine.|
|Detected analyte||Diethylstilbestrol (DES) in tissue and urine.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|