EuroProxima Domoic Acid is a competitive enzyme immunoassay for quantitative analysis of domoic acid in scallop, mussel and oyster samples.
High concentrations of shellfish toxins can accumulate in filter feeders such as clams, mussels and oysters causing illness amongst people who eat them. There are four syndromes called shellfish poisoning, i.e paralytic shellfish poisoning (PSP), neurotoxic shellfish poisoning (NSP), diarrhetic shellfish poisoning (DSP) and amnesic shellfish poisoning (ASP). ASP is caused by domoic acid (DA) which is produced by the phytoplankton Pseudo-nitzschia. DA is an excitatory amino acid containing the structure of glutamic acid and resembling kainic acid.
In the European Union, Regulation (EC) No 853/2004 stipulates that live bivalve mollusks must not contain DA in total quantity (measured in the whole body or any part edible separately) that exceeds a limit of 20 mg/kg (ppt) DA.
The EuroProxima Domoic acid ELISA is a competitive enzyme immunoassay based on antibodies directed against domoic acid.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each)|
|Sample preparation||For mentioned matrices fast and efficient extraction methods are included in the kit description.|
|Incubation time||45 min (20 – 25 °C)|
|LOD (Detection limit)||Scallop 23 µg/kg, mussel 21 µg/kg, oyster 23 µg/kg|
|Detection capability CCβ||Scallop/mussel 60 µg/kg, oyster 150 µg/kg|
|Validated matrices||Scallop, mussel and oyster.|
|Detected analyte||Domoic acid in scallop, mussel and oyster.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|