EuroProxima Ethinylestradiol is a competitive enzyme immunoassay (ELISA) for screening and quantitative analysis of ethinylestradiol in tissue, plasma/serum and urine.
Ethinylestradiol is a synthetic sexual hormone which is, like all other natural or synthetic steroid hormones, prohibited for use as resource in fattening in the European Community since 1988. It is a synthetic alkylated estradiol with a 17α-ethinyl substitution. It has high estrogenic potency when administered orally and is often used as the estrogenic component in oral contraceptives.
- The sensitivity of this ELISA is far below the maximum residue limits as fixed by the European Union: 100 μg/kg (ppb) in fat and liver and 30 μg/kg in kidney
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each).|
|Sample preparation||For mentioned matrices fast and efficient extraction methods are included in the kit description.|
|Incubation time||2 h 30 min (4 °C / 20 – 25 °C)|
|LOD (Detection limit)||Tissue 0.18 ng/ml (ppb), tissue (SPE) 0.07 ng/ml, plasma/serum 0.14 ng/ml, urine (SPE) 0.33 ng/ml|
|Detection capability CCβ||Tissue 0.2 ng/ml, tissue (SPE) 0.2 ng/ml, plasma/serum 0.5 ng/ml, urine (SPE) 0.5 ng/ml|
|Validated matrices||Tissue, plasma/serum and urine.|
|Detected analyte||Ethinylestradiol in tissue, plasma/serum and urine.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|