A competitive enzyme immunoassay for screening and quantitative analysis of florfenicol-amine in various matrices.
Florfenicol (FF) is an effective broad-spectrum antibiotic and an analogue of chloramphenicol (CAP). Due to the ban of the use of CAP in food-producing animals, FF is used to control infections in humans, pigs, poultry, non-ruminating cattle and aquaculture. Within the EU, Maximum Residue Limits (MRLs) for FF have been established. These MRLs are described as: “Sum of florfenicol and its metabolites measured as florfenicol-amine”. For this reason, EuroProxima has developed an ELISA that is specific for the measurement of florfenicol-amine.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each)|
|Sample preparation||For the mentioned matrices, fast and efficient methods are included in the test kit description.|
|Incubation time||1 h 30 min|
|LOD (Detection limit)||Tissue 5.1 µg/kg (ppb), fish 8.3 µg/kg, kidney 11.6 µg/kg, liver 7.4 µg/kg, milk 2.1 µg/l, egg 5.0 µg/kg|
|Detection capability CCβ||Tissue 15 µg/kg, fish 15 µg/kg, kidney 15 µg/kg, liver 15 µg/kg, milk 8 µg/l, egg 8 µg/kg|
|Validated matrices||Tissue, fish, kidney, liver, milk and egg.|
|Detected analyte||Florfenicol-amine in various matrices.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|