RIDASCREEN® Tetracyclin is a competitive enzyme immunoassay for the quantitative analysis of tetracyclins in milk, milk powder, cheese, butter, dairy products (curd, yoghurt (plain/with fruits), kefir, cream, sour cream) honey, meat, sausage, fish, shrimp and whole egg.
1948 aureomycin (chlortetracycline) was isolated by Duggan as a metabolite of the actinomyces species streptomyces aureofaciens. This was the first antibiotic substance of the group of tetracyclines. In Germany tetracycline, chlortetracycline and oxytetracycline are authorized for veterinary use. Therefore health risks for the consumer can occur.
In all animal species which are used for food production tetracycline residues (the sum of mother substance and the 4-epimer) are limited in the EU law, commission regulation (EU) No 37/2010 with the following limits: 100 µg/kg (ppb) in muscle and in milk.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each)|
|Sample preparation||For the mentioned matrices fast and efficient methods are included in the test kit description, see under 9. preparation of samples.|
|Incubation time||1 h 30 min|
|LOD (Detection limit)||Milk approx. 0.9 μg/l, milk powder approx. 5 μg/kg, cheese approx. 2.3 μg/kg, butter approx. 2.6 μg/kg, dairy products approx. 1 μg/kg, honey approx. 3.7 μg/kg, meat approx. 1.5 μg/kg, sausage approx. 4.6 μg/kg, fish approx. 1.5 μg/kg, shrimp approx. 1.2 μg/kg, whole egg approx. 2.8 μg/kg|
|Validated matrices||Milk, milk powder, cheese, butter, dairy products, honey, meat, sausage, fish, shrimp, whole egg.|
|Detected analyte||Tetracyclins in milk, milk powder, cheese, butter, dairy products (curd, yoghurt (plain/with fruits), kefir, cream, sour cream) honey, meat, sausage, fish, shrimp and whole egg.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|