RIDASCREEN® Streptomycin is a competitive enzyme immunoassay for the quantitative analysis of streptomycin in milk and milk powder, honey, meat, liver, kidney, shrimp and apple juice.
Besides β-lactam antibiotics in veterinary medicine streptomycin is one of the mostly used antibiotics for the treatment of mastitis. Residues of streptomycin may therefore occur in food of animal origin, if the withholding period is not obeyed or if it is used improperly. High concentrations of streptomycin show ototoxic and nephrotoxic effects. The chronic exposure of humans with low concentrations, as found in food, may cause allergies, impair the intestinal flora and induce resistance of pathogenic microorganisms. To protect the consumer against health risks and avoid food-technological problems, a sensitive and simple method for the detection of streptomycin is necessary. The EU regulations for streptomycin are fixed in MRLs (maximum residue limits) for meat and milk (muscle and liver: 500 μg/kg (ppb), kidney: 1000 μg/kg and milk: 200 μg/l). In honey, the EU Community Reference Laboratories have determined a zero-tolerance with a minimum required performance limit of ccβ = 40 μg/kg for test systems.
- Broad range of matrices: milk and milk powder, honey, meat, liver, kidney, shrimp and apple juice.
- Improved sensitivity.
- Short test: 45min.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each).|
|Sample preparation||Milk: dilution, milk powder: reconstitution, dilution
Honey: extraction, chromatographic clean-up with RIDA® C18 column, evaporation, reconstitution
Meat, liver, kidney, shrimp: homogenization, extraction, dilution
Apple juice: centrifugation, dilution
|Incubation time||45 min|
|LOD (Detection limit)||Milk approx. 5 μg/l (ppb), milk powder (reconstituted from milk powder) approx. 3 μg/kg, honey approx. 2 μg /kg, beff, pork approx. 22 μg/kg, poultry approx. 28 μg/kg, liver approx. 23 μg/kg, kidney approx. 18 μg/kg, shrimp approx. 20 μg/kg, apple juice approx. 4 μg/l|
|Detection capability CCβ||Milk 10 µg/l, milk powder µg/kg, honey 5 µg/kg, beef, pork 50 µg/kg, poultry 50 µg/kg, liver 50 µg/kg, kidney 50 µg/kg, shrimp 50 µg/kg, apple juice 10 µg/l|
|Validated matrices||Milk, milk powder, honey, meat, liver, kidney, shrimp and apple juice.|
|Detected analyte||Streptomycin in milk and milk powder, honey, meat, liver, kidney, shrimp and apple juice.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|