RIDASCREEN® Sulfamethazin is a competitive enzyme immunoassay for the quantitative determination of sulfamethazine in milk, meat, honey, liver, kidney, fish, shrimp and egg.
Sulfonamides are widely used as feed additives, mainly for fattening of calves and pigs. Combined with inhibitors of dihydrofolate reductase such as trimethoprim, tetroxoprim, or pyrimethamine sulfonamides are also used in veterinary medicine for the treatment of intestinal infections, mastitis, pneumonitis and other (systemic) diseases. Sulfonamide residues may therefore occur in food of animal origin such as meat and milk. Particularly the transmission of the carcinogenic sulfamethazine represents a threat to human health. The EU established a maximum residue limit (MRL) for all substances of the sulfonamide-group of 100 μg/kg (ppb) in muscle, fat, liver, kidney and milk.
- High specificity for sulfamethazine only: No significant cross reactivity to other sulfonamides.
- Shortened test implementation of 45 min only.
- Improved sensitivity and recovery.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each).|
|Sample preparation||Milk: direct use, meat: homogenization, centrifugation
Honey: extraction, clean-up with RIDA® C18 column, evaporation, reconstitution, precipitation
Liver, kidney, fish: homogenization, centrifugation, precipitation
Shrimp, egg: homogenization, extraction, evaporation, reconstitution
|Incubation time||45 min|
|LOD (Detection limit)||Milk approx. 4 μg/l, meat (bovine, porcine) approx. 5 μg/kg, meat (poultry) approx. 10 μg/kg, honey approx. 10 μg/kg*, liver approx. 6 μg/kg, kidney approx. 10 μg/kg, fish approx. 7 μg/kg, shrimp approx. 15 μg/kg, egg approx. 16 μg/kg
*For honey the LOD can deviate due to matrix effects.
|Detection capability CCβ||Milk 10 µg/l, meat (bovine, porcine) 10 µg/kg, meat (poultry) 15 µg/kg, honey 20 µg/kg, liver 15 µg/kg, kidney 15 µg/kg, fish 20 µg/kg, shrimp 20 µg/kg, egg 15 µg/kg|
|Validated matrices||Milk, meat (bovine, porcine), meat (poultry), honey, liver, kidney, fish, shrimp and egg.|
|Detected analyte||Sulfamethazine in milk, meat, honey, liver, kidney, fish, shrimp and egg.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|