EuroProxima Fluoroquinolones is a competitive enzyme immunoassay for screening and quantitative analysis of a broad range of fluoroquinolones in various matrices.
Fluoroquinolones are a synthetic class of antibiotics which all act by inhibition of bacterial DNA-gyrase. The antiserum used in the ELISA is directed against the main fluoroquinolones including enrofloxacin and its metabolite ciprofloxacin. In combination with our specific ELISA against flumequine (5101FLUM) nearly all fluoroquinolones can be screened for in a fast and reliable way.
The Fluoroquinolones ELISA is a competitive enzyme immunoassay based on antibodies directed against fluoroquinolones.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each).|
|Sample preparation||For the mentioned matrices, fast and efficient methods are included in the test kit description.|
|Incubation time||1 h 30 min|
|LOD (Detection limit)||Tissue 0.3 ng/g (ppb), egg 6 ng/g, tissue and whole egg 0.5 ng/g, milk 3 ng/ml, water 2 ng/ml, honey 2 ng/g, serum 2.5 ng/ml, urine 7 ng/ml|
|Validated matrices||Tissue, egg, tissue and whole egg, milk, water, honey, serum and urine.|
|Detected analyte||Fluoroquinolones in tissue, egg, tissue and whole egg, milk, water, honey, serum and urine.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|