A competitive enzyme immunoassay for screening and quantitative analysis of gentamicin in various matrices.
Gentamicin belongs to a group of carbohydrate containing antibiotics called aminoglycosides. All the aminoglycosides are potentially toxic compounds causing significant damage in vestibular and auditory functions in humans as well as in animals. Within the EU, MRLs have been established for gentamicin residues in bovine milk (100 µg/l (ppb)), bovine/porcine muscle (50 µg/kg), fat (50 µg/kg), liver (200 µg/kg) and kidney (750 µg/kg). In combination with the streptomycin and neomycin ELISA we offer an unique panel for the detection of these three aminoglycosides in a range of matrices.
The gentamicin ELISA is a competitive enzyme immunoassay based on antibodies directed against protein conjugated gentamicin.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each)|
|Sample preparation||For the mentioned matrices, fast and efficient methods are included in the test kit description.|
|Incubation time||1 h 30 min|
|LOD (Detection limit)||Milk 0.4 µg/l , tissue 12.5 µg/kg , serum 2 µg/l, honey 5 µg/kg , egg 1 µg/kg, urine 4 µg/l, feed 10 µg/kg|
|Detection capability CCβ||Milk 10 µg/l|
|Validated matrices||Milk, tissue, serum, honey, egg, urine and feed.|
|Detected analyte||Gentamicin in various matrices.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|