EuroProxima Malachite Green Total is a competitive enzyme immunoassay for quantitative analysis of malachite green, leucomalachite green, crystal violet and leucocrystal violet in fish and shrimp.
Malachite green (MG) and crystal violet (CV) are organic dyes used in the aquaculture industry as effective fungicides and disinfectants. Due to their toxic effects they are not authorised to be used in fish for human consumption. These dyes are in vivo metabolised to the reduced forms leucomalachite green (LMG) and leucocrystal violet (LCV). Within the EU, a Minimum Required Performance Limit (MRPL) of 2 µg/kg (ppb) is set for the sum of MG and CV.
The EuroProxima Malachite Green Total ELISA is a competitive enzyme immunoassay for the quantitative analysis of MG, LMG, CV and LCV in fish and shrimp.
- Simple and fast sample preparation method
- Fully validated for MG, LMG, CV and LCV using 7 species of fish and shrimp
- Detection capability for each analyte determined in fish and shrimp matrices
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each)|
|Sample preparation||For the mentioned matrices, fast and efficient methods are included in the test kit description.|
|Incubation time||1 h|
|LOD (Detection limit)||Fish and shrimp 0.12 ng/g (ppb)|
|Detection capability CCβ||Fish and shrimp MG 0.3 ng/g, fish and shrimp LMG 0.3 ng/g, fish and shrimp CV 0.3 ng/g, fish and shrimp LCV 0.3 ng/g, fish and shrimp MG + LMG 0.3 ng/g, fish and shrimp CV + LCV 0.3 ng/g|
|Validated matrices||Fish and shrimp.|
|Detected analyte||Malachite green, leucomalachite green, crystal violet and leucocrystal violet in fish and shrimp.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|