Description
Intended use:
RIDASCREEN®FAST β-Lactoglobulin is a sandwich enzyme mmunoassay for the quantitative analysis of native and processed β-lactoglobulin in rice crispies, chocolate and sausage.
General Information:
Cow’s milk contains 3.2 % proteins which consist of 10 % β-lactoglobulin (leading protein of whey) and 80 % caseins. The most important allergen especially for children is β-lactoglobulin. For adults the caseins become more dominant. Milk can induce allergic reactions at infancy. Whey or milk powder is often added to food products, therefore it is recommended to determine β-lactoglobulin in food. The allergen can be present as an ingredient or as a contamination in raw and cooked products. According to the regulation (EU) No. 1169/2011, milk and products thereof must be declared on food labels. Similar regulations exist e.g. in the USA, Canada, Australia and New Zealand.
Benefits:
- Fast: Only 30 minutes incubation time during procedure (by far the fastest compared to competitors).
- Specificity: no cross reactivities have been observed in a wide range of matrices (85).
- Validated for automates.
Accessories:
| Article Numbers | R4912 |
|---|---|
| Test format | Microtiter plate with 48 wells (6 strips with 8 removable wells each) |
| Sample preparation | Homogenization and extraction |
| Incubation time | 30 min Room temperature |
| LOD (Detection limit) | 0.042 mg / kg (ppm) ß-lactoglobulin (mean) 0.024 – 0.073 mg / kg (ppm) ß-lactoglobulin* *depending on matrix |
| LOQ (Limit of quantification) | 0.167 mg / kg (ppm) ß-lactoglobulin |
| Cross reactivity | There is a cross reactivity to sheep’s, goat’s and buffalo’s milk. |
| Validated matrices | Rice crispies, chocolate and sausage. |
| Available application notes |
|
| Detected analyte | β-lactoglobulin of cow’s milk |
| Evaluation | Microtiter plate spectrophotometer (450 nm) |
| Instructions | |
|---|---|
| MSDS |
