EuroProxima Okadaic Acid is a competitive enzyme immunoassay for quantitative analysis of domoic acid in scallop, mussel and oyster samples.
High concentrations of shellfish toxins can accumulate in filter feeders such as clams, mussels, and oysters causing illness amongst people who eat them. There are four syndromes called shellfish poisoning, i.e paralytic shellfish poisoning (PSP), neurotoxic shellfish poisoning (NSP), diarrhetic shellfish poisoning (DSP) and amnesic shellfish poisoning (ASP). DSP is primarily caused by the polyether toxin okadaic acid (OA) and several analogues of OA, among which the dinophysistoxins DTX1, DTX2, and DTX3.
In the European Union, Regulation (EC) No 853/2004 stipulates that live bivalve mollusks must not contain OA in total quantity (measured in the whole body or any part edible separately) that exceeds a limit of 160 μg OA equivalents (OA, dinophysistoxins, and pectenotoxins together) per kilogram (ppb).
The EuroProxima Okadaic Acid ELISA is a competitive enzyme immunoassay based on antibodies directed against okadaic acid.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each).|
|Sample preparation||For mentioned matrices fast and efficient extraction methods are included in the kit description.|
|Incubation time||45 min (20 – 25 °C)|
|LOD (Detection limit)||Mussel/oyster 50 µg/kg (ppb)|
|Detection capability CCβ||Mussel/oyster 40 µg/kg|
|Validated matrices||Mussel and oysters.|
|Detected analyte||Okadaic acid in mussel and oyster samples.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|