EuroProxima Tylosin is a competitive enzyme immunoassay for screening and quantitative analysis of tylosin in various matrices.
Tylosin is a macrolide antibiotic that is active against Gram-positive bacteria and mycoplasmas. It is used in pigs, cattle and poultry for the treatment of conditions caused by sensitive organisms. In beekeeping tylosin is used to prevent infection diseases like American foulbrood. Tylosin consists predominantly of tylosin A and varying amounts of tylosin B, tylosin C and tylosin D. In the past, tylosin has been used as a feed additive in animal feed to promote growth, but in 1998 it was banned in accordance with Council Regulation (EC) no. 2821/98.
The Tylosin ELISA is a competitive enzyme immunoassay based on antibodies directed against tylosin.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each).|
|Sample preparation||For the mentioned matrices, fast and efficient methods are included in the test kit description.|
|Incubation time||1 h 15 min|
|LOD (Detection limit)||Milk 2.5 ng/ml (ppb), honey 2.5 ng/ml , egg 2.5 ng/ml, tissue 2.5 ng/ml , serum 2.5 ng/ml, urine 2.5 ng/ml, feed 2.5 ng/ml|
|Validated matrices||Milk, honey, egg, tissue, serum, urine and feed.|
|Detected analyte||Tylosin in various matrices.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|