RIDASCREEN® Egg is a sandwich enzyme immunoassay developed for the quantitative analysis of native and processed hen´s egg in foods. The following foods were examined as representatives of the group of products including baked goods, sweets, sauces, and beverages: ice cream, noodles, salad dressing, wine, chocolate, and cookies. It is expected that this test will also be suited for the analysis of other foods; this needs to be checked by the user. The detection limit and the limit of quantification depend on the respective sample matrix, the extent of processing, and the extraction method.
Egg protein can either be present as an ingredient or as contamination in raw or heated foods. Regulation (EU) No. 1169/2011 prescribes that egg must be listed on food labels as a cause of food allergies. Comparable legal regulations are in place in the U.S.A., Canada, Australia, China, New Zealand, and many other countries.
Egg white contains approx. 10 % – 11 % protein. Allergologically of importance are four main allergens that make up 80 % of egg white protein content. The main allergens include ovomucoid (11 %), ovalbumin (54 %), ovotransferrin (12 %), and lysozyme (3.5 %). In contrast, the proteins in the egg yolk have only moderate allergenicity.
- Detect processed and native egg in food matrix.
- Two different extraction methods in one kit for the detection of processed egg with A-AEP or the easier extraction for native egg with the AEP.
- AOAC-RI validation (PTM) in preparation.
|Test format||Microtiter plate with 96 wells (12 strips with 8 removable wells each)|
|Sample preparation||Homogenization and extraction|
|Incubation time||50 min
|LOD (Detection limit)||0.15 mg / kg (ppm) whole egg powder (mean)
0.08 – 0.27 mg / kg (ppm) whole egg powder*
*depending on matrix
|LOQ (Limit of quantification)||0.25 mg / kg (ppm) whole egg powder
equivalent to 0.12 mg / kg (ppm) whole egg protein
|Cross reactivity||Cross reactivity is given to egg from gooses, quails and ostrich.|
|Validated matrices||Ice cream, noodles, salad dressing, wine, chocolate, and cookies.|
|Detected analyte||Egg white proteins ovalbumin and ovomucoid from hen´s egg.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|