RIDASCREEN® Fumonisin is a competitive enzyme immunoassay for the quantitative determination of fumonisin in corn and corn products (corn flour, corn, semolina, polenta, corn for popcorn).
Fumonisins are carcinogenic, neuro-, hepato- and pneumotoxic metabolites of molds produced by some Fusarium species. The doses of fumonisin sufficient to induce toxic effects differ significantly depending on the animal species. A concentration of approx. 5 – 10 mg/kg (ppm) fumonisin in feed induces neuro-toxic effects in horses. In pigs the ingestion of 4 – 16 mg/kg body weight may result in liver cirrhosis and more than 16 mg/kg bw. may lead to pulmonary edema. Chickens tolerate higher concentrations of fumonisin in feed, up to 75 mg/kg. Cattle seem to be insensitive to high fumonisin concentrations.
- The low detection limit of this tests allows to also identify low fumonisin concentrations.
- Up to 42 samples can be tested in double-determination.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each)|
|Sample preparation||Extraction, filtration and dilution.|
|Incubation time||45 min|
|LOD (Detection limit)||25 µg/kg (ppb)|
|Validated matrices||Corn and corn products (corn flour, corn semolina, polenta and popcorn).|
|Available application notes||Please contact your local R-Biopharm distributor for further information and application.|
|Detection range||0.025 – 2 mg/kg|
|Detected analyte||Fumonisin in corn and corn products (corn flour, corn, semolina, polenta, corn for popcorn).|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|