RIDASCREEN®FAST Folic acid is a competitive enzyme immunoassay for the quantitative determination of added folic acid in milk, milk powder, food for special medical purpose, grain and cereals, fortified flour, vitamin powder, -mixture, -tablets and vitamin juice.
Folic acid also known as vitamin B9 or vitamin B11 belongs to the group of water soluble vitamins. It can´t be produced by the human body and therefore must be included in the diet in order to meet the daily requirements. Folic acid plays a key role in all growth and cell division processes. Adequate folic acid intake during pregnancy is important to avoid neural tube defects, for example. Some countries have agreed to fortify either bread, cereals, flour, pasta, rice or other grain products with folic acid in different concentrations as a preventive approach to improve micronutrient status of the population.
- Low cross reactivity for a large number of folic acid derivatives.
- The results are available within 1 h, so the test is very fast and suitable for in-process and finalproduct control.
- No additional reagents are required with the exception of distilled / demineralized water.
|Test format||Microtiter plate with 48 wells (6 strips with 8 removable wells each)|
|Sample preparation||Milk: can be applied directly, dilution if necessary.
Juices: can be applied directly, dilution if necessary.
Other matrices: extraction, dilution if necessary.
|Incubation time||25 min|
|LOD (Detection limit)||0.5 μg/L (ppb)|
|Cross reactivity||Folic acid: 100 %
Dihydrofolic acid: approx. 14 %
Tetrahydrofolic acid: < 0.5 %
5-Methyltetrahydrofolic acid: < 0.5 %
Formyltetrahydrofolic acid: < 0.5 %
Tetrahydrobiopterin:< 0.5 %
6-Biopterin: < 0.5 %
|Validated matrices||Milk, milk powder, grain and cereals, fortified flour, vitamin powder, -mixture, -tablets and vitamin juice.|
|Detected analyte||Folic acid concentration|
|Evaluation||Microtiter plate photometer 450 nm|