RIDASCREEN®FAST Mustard is a sandwich enzyme immunoassay for the quantitative analysis of mustard in ketchup, sausage and cream cheese.
People allergic to mustard will react to any ingredient of black, Indian brown or yellow / white mustard. Even small traces of allergens in food can trigger allergic reactions in sensitive individuals. Mustard is used as a condiment in meat, to prepare pastes or as an ingredient for a large variety of sauces, soups, salad dressings, and marinades. Thus, it is frequently encountered in food as a hidden allergen. Food manufacturers have to check for crosscontamination. The allergen can be present as an ingredient or as a contamination in raw and cooked products. According to the regulation (EU) No. 1169/2011, mustard and products thereof must be declared on food labels.
- One of the most sensitive musard ELISAs on the market.
- Widespread testing for cross reactivity (81 matrices have been evaluated).
|Test format||Microtiter plate with 48 wells (6 strips with 8 removable wells each)|
|Sample preparation||Homogenization, extraction and centrifugation|
|Incubation time||30 min
|LOD (Detection limit)||0.1 mg / kg (ppm) mustard flour (mean)
0.08 – 0.11 mg / kg (ppm) mustard flour*
*depending on the matrix
|LOQ (Limit of quantification)||0.5 mg / kg (ppm) mustard flour|
|Cross reactivity||Cross reactivity to kidney beans, pinto beans, white beans, linseed as well as rapeseed and seeds from different cruciferous plants (Brassicaceae).|
|Validated matrices||Ketchup, sausage and cream cheese.|
|Detected analyte||Different kinds of mustard (yellow, white, brown, black mustard).|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|