The SureFood® ALLERGEN 4plex Soya/Celery/Mustard+IAC is a real-time PCR for the direct, qualitative detection and differentiation of specific celery (Apium graveolens), mustard (Brassica carinata, Brassica juncea, Brassica nigra, Sinapis alba, Sinapis arvensis) and soya (Glycine max) DNA sequences according to directive (EC) 1169/2011. Each reaction contains an internal amplification control (IAC).
For DNA preparation, the use of SureFood® PREP Advanced (S1053), protocol 1, is recommended.
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical validation of instruments was performed on Roche LightCycler® 480 II, Qiagen Rotor-Gene Q, Agilent Aria Dx, Applied Biosystems 7500, Bio-Rad CFX96, R-Biopharm RIDA®CYCLER and Agilent Mx3005P.
Watch our video tutorials on sample preparation and test procedure with SureFood® ALLERGEN:
|Test format||100 reactions|
|LOD (Detection limit)||≤ 0.4 mg / kg|
|Evaluation||Real-time PCR instrument with four detection channels (510 nm, 580 nm, 610 nm and 660 nm)|