Description
Intended use:
The real-time PCR test detects DNA of sesame (Sesamum indicum) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC).
General Information:
For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 (S3301) containing 40 mg gluten / kg food sample and a detection system including a standard dilution is required.
For DNA preparation, the use of SureFood® PREP Advanced (S1053), protocol 1, is recommended.
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC / HEX at the same time. The technical validation of instruments was performed on Roche LightCycler® 2.0, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC, LTF MyGo Pro, Agilent AriaDx and Agilent Mx3005P.
The method is validated for quantitative determination of 1 mg to 400 mg allergenic substance / kg food sample using the reference material SureFood® QUANTARD Allergen 40 containing 40 mg allergenic substance / kg food sample.
Accessories:
| Article Numbers | S3608 |
|---|---|
| Test format | 100 reactions |
| LOD (Detection limit) | ≤ 0.4 mg / kg |
| LOQ (Limit of quantification) | 1 mg / kg using SureFood® PREP Advanced, protocol 1 |
| Cross reactivity | Cross reactivity was observed with DNA extracts from devil´s claw (Harpagophytum procumbens) with 100 %. |
| Evaluation | Real-time PCR instrument with two detection channels (510 nm and 580 nm) |
| Instructions | |
|---|---|
| MSDS |
