The SureFood® GMO QUANT MON863 Corn real-time PCR test detects the relative quantitative MON863 Corn DNA amount.
Therefore the kit contains two PCR systems, one specific for the MON863 Corn (OECD unique identifier MON-ØØ863-5) and the other one specific for corn (the reference gene). The event specific MON863 Corn detection is according to the official method of the European Commission.
For DNA-preparation of raw material the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.
The real-time PCR assay can be used with established real-time PCR instruments (Roche LightCycler®, Rotor-Gene Q, ABI PRISM, Eppendorf realplex, BioRad CFX96, Agilent MxSeries etc.).
|Test format||2 x 50 reactions|
|LOD (Detection limit)||The MON863 Corn PCR has a limit of detection of ≤ 5 DNA-copies. The assay limit of detection depends on sample matrix, processing grade, DNA-preparation and DNA-content.
The limit of quantitation depends on the concentration of the sample DNA used in the analysis. For example, if 50,000 target-sequence copies of corn specific reference gene are present, the relative quantitation limit for MON863 Corn DNA is 0.1 %.