Description
Intended use:
VitaFast® Vitamin C (L-Ascorbic Acid) is a test in microtiter plate format for the quantitative determination of vitamin C (L-ascorbic acid) in foods, pharmaceutical products and other sample materials. Furthermore the total amount of vitamin C (L-ascorbic acid + L-dehydroascorbic acid) can be detected (see application note).
General Information:
Ascorbic acid is a clear, odourless, cristalline solid substance which has a sour flavour. There are four stereoisomeric variants but only L-(+)-ascorbic acid (also referred to as vitamin C) is biologically active. Its vitamin activity is the most relevant property of L-ascorbic acid: humans cannot produce vitamin C and require it as part of their nutrition. A deficiency can result in the manifestation of scurvy. L-ascorbic acid and its salt, the anion L-ascorbate, will hereinafter be referred to as ascorbic acid and ascorbate, respectively.
Benefits:
- The method is robust and suitable for the routine determination of the ascorbic acid content in a multitude of foodstuffs and beverages.
- This test has very low detection and quantification limit.
| Article Numbers | P1010 |
|---|---|
| Test format | Microtiter plate with 96 wells (12 strips with 8 removable wells each) |
| Sample preparation | Homogenisation, extraction, filtration, dilution, |
| Incubation time | Step 1 = 20 min in the dark at 20-25 °C, Step 2 = 30 min in the dark at 20-25 °C. |
| LOD (Detection limit) | 7.8 mg / 100 g (ml) |
| LOQ (Limit of quantification) | 7.8 mg / 100 g (ml) |
| Validated matrices | Fruit gums, multivitamin effervescent tablets, Iron and vitamin C tablets. |
| Available application notes | Total amount of vitamin C (L-ascorbic acid + L-dehydroascorbic acid) |
| Detected analyte | Ascorbic acid concentration |
| Evaluation | Microtiter plate photometer 550-570 nm |
| Instructions | |
|---|---|
| MSDS |
