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Γάτα & Σκύλος (Cat & Dog)

SureFood® ANIMAL ID Cat & Dog IAAC

Art. No.: S6112

Intended use:

The test detects cat (Felis catus) DNA and dog (Canis lupus familiaris) DNA seperately. Each reaction contains an internal amplification control and an internal detection assay for animal DNA (IAAC).

For DNA-preparation of raw material the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.

General information:

The real-time PCR assay can be used with established realtime PCR instruments, equipped for detection of two fluorescence emissions at 522 nm and 553 nm at the same time. The technicalvalidation of instruments was performed on Roche LightCycler® 2.0*, Roche LightCycler® 480 II, Qiagen Rotor-Gene Q, Applied Biossystems 7500, Bio-Rad CFX96 and Agilent Mx3005P.

*note: A Color Compensation is necessary for users of the Roche LightCyclerR 2.0. The SureCC Color Compensation kit II (Art. No. F4010) must be used for the color compensation of such devices.

Specifications
Art. No. S6112
Test Format 2 x 50 reactions
Sample Preparation 1. DNA preparation, e.g. SureFood® PREP ANIMAL PREP or ANIMAL PREP X
2. Preparation of Allergen-PCR mix
3. Pipetting of samples and PCR mix
4. Check PCR protocol settings
5. Start PCR run
Detection Limit The SureFood® ANIMAL ID Cat & Dog IAAC real-time PCR is developed for the detection of cat and dog DNA in a DNA mixture at a relative amount of 0.5 %. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.
Shelf life 12 month
Additional Information The detection system system can be used on all available real-time PCR cyclers.ABI Prism
Eppendorf realplex
iCycler
Rotorgene
LightCycler

 

Animal Identification

DNA Preparation
Beef
Pork
Chicken / Turkey
Cat & Dog
Horse & Donkey
Fish
Rabbit