Γάτα & Σκύλος (Cat & Dog)
SureFood® ANIMAL ID Cat & Dog IAAC
The test detects cat (Felis catus) DNA and dog (Canis lupus familiaris) DNA seperately. Each reaction contains an internal amplification control and an internal detection assay for animal DNA (IAAC).
The real-time PCR assay can be used with established realtime PCR instruments, equipped for detection of two fluorescence emissions at 522 nm and 553 nm at the same time. The technicalvalidation of instruments was performed on Roche LightCycler® 2.0*, Roche LightCycler® 480 II, Qiagen Rotor-Gene Q, Applied Biossystems 7500, Bio-Rad CFX96 and Agilent Mx3005P.
*note: A Color Compensation is necessary for users of the Roche LightCyclerR 2.0. The SureCC Color Compensation kit II (Art. No. F4010) must be used for the color compensation of such devices.
|Test Format||2 x 50 reactions|
|Sample Preparation||1. DNA preparation, e.g. SureFood® PREP ANIMAL PREP or ANIMAL PREP X
2. Preparation of Allergen-PCR mix
3. Pipetting of samples and PCR mix
4. Check PCR protocol settings
5. Start PCR run
|Detection Limit||The SureFood® ANIMAL ID Cat & Dog IAAC real-time PCR is developed for the detection of cat and dog DNA in a DNA mixture at a relative amount of 0.5 %. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.|
|Shelf life||12 month|
|Additional Information||The detection system system can be used on all available real-time PCR cyclers.ABI Prism