Βοδινό (Beef)
SureFood® ANIMAL ID Beef IAAC
Intended use:
The test detects beef DNA (Bos taurus). Each reaction contains an internal amplification control and an internal detection assay for animal DNA (IAAC).
For DNA-preparation of raw material the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.
General information:
The real-time PCR assay can be used with established realtime PCR instruments, equipped for detection of two fluorescence emissions at 522 nm and 553 nm at the same time. The technical validation of instruments was performed on Roche LightCycler® 2.0*, Roche LightCycler® 480 II, Qiagen Rotor-Gene Q, Applied Biossystems 7500, Bio-Rad CFX96 and Agilent Mx3005P.
| Art. No. | S6113 |
|---|---|
| Test format | 100 reactions |
| Detection limit | The SureFood® ANIMAL ID Beef IAAC real-time PCR is developed for the detection of beef DNA in muscle meat mixture at a relative amount of 0.1 %. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content. |
| Shelf life | 12 month |
|---|---|
| Additional Information | The detection system system can be used on all available real-time PCR cyclers. (ABI, Prism ,Eppendorf realplexi Cycler, Rotorgene LightCycler). |
SureFood® Animal QUANT Beef
Art. No.: S1010
Intended use:
The test detects the relative beef DNA amount to the total animal DNA content in meat samples. Therefore the kit contains two PCR systems, one for detection of a beef specific gene (Bos detection gene) and one for the detection of an animal gene (Ref reference gene).
The use of SureFood® PREP Basic is recomended.
General information:
The real-time PCR assay can be used with established real-time PCR instruments (Roche LightCycler®, Rotor-Gene Q, ABI PRISM, Eppendorf realplex, BioRad CFX96, Agilent MxSeries etc.).
*note: A Color Compensation is necessary for users of the Roche LightCyclerR 2.0. The SureCC Color Compensation kit II (Art. No. F4010) must be used for the color compensation of such devices.
| Art. No. | S1010 |
|---|---|
| Test format | 2 x 50 reactions |
| Detection limit | The beef specific PCR has a limit of detection of ≤ 5 DNA-copies. The assay limit of detection depends on sample matrix, processing grade, DNA-preparation and DNA-content. The limit of quantitation depends on the concentration of the sample DNA used in the analysis. For example, if 50,000 target-sequence copies of the reference gene are present, the relative quantitation limit for beef DNA is 0.04 %. |
| Cross reactivity | Cross reactivity was observed with DNA extracts from Bison sp. (100 %) and Water buffalo (Bubalis arnee) with 0.6%. |
SureFood® ANIMAL ID 4plex Beef/Sheep/Goat+IAAC
Intended use:
The test detects beef (Bos taurus), sheep (Ovis aries) and goat (Capra hircus) DNA. Each reaction contains an internal amplification control and an internal detection assay for animal DNA (IAAC).
For DNA-preparation the use of SureFood® PREP Basic is recomended.
General information:
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of four fluorescence emissions at 510 nm, 580 nm, 610 nm and 680 nm at the same time. The real-time PCR assay can be used with established real-time PCR instruments (Roche LightCycler® 480*, Qiagen Rotor-Gene Q, ABI PRISM, Bio-Rad CFX96, Agilent MxSeries etc.).
*note: A Color Compensation is necessary for users of the Roche LightCycler® 2.0. The SureCC Color Compensation kit II (Art. No. F4010) must be used for the color compensation of such devices.
| Art. No. | S6121 |
|---|---|
| Test format | 100 reactions |
| Detection limit | The SureFood® ANIMAL ID 4plex Beef/Sheep/Goat+IAAC real-time PCR is developed for the detection of beef, sheep and goat DNA in muscle meat mixture at a relative amount of 0.1 %. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content. |
| Shelf life | 12 Months |
SureFood ® ANIMAL ID 4plex Beef/Horse/Pork+IAAC – NEW
Art. No.: S6126
Intended use:
The test detects beef (Bos taurus), horse (Equus caballus) and pork (Sus scrofa) DNA. Each reaction contains an internal amplification control and an internal detection assay for animal DNA (IAAC).
General Information:
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of four fluorescence emissions at 522 nm, 553 nm, 610 nm and 670 nm (FAM, VIC, ROX and Cy5) at the same time. The technical validation of instruments was performed on Roche LightCycler® 480 II/Roche cobas z 480 Analyzer2, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, BMS MIC, Agilent Mx3005P and Agilent AriaDx.
Accessories:
| Art. No. | S6126 |
|---|---|
| Test format | 100 reactions |
| Sample preparation | For DNA-preparation the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended. |
| Detection limit | The SureFood® ANIMAL ID 4plex Beef/Horse/Pork+IAAC real-time PCR is developed for the detection of beef, horse and pork DNA in muscle meat mixture at a relative amount of 0.5 %. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content. |
| Shelf life | 12 Months |
Animal Identification
DNA Preparation
Beef
Pork
Chicken / Turkey
Cat & Dog
Horse & Donkey
Fish
Rabbit