EuroProxima Erythromycin is a competitive enzyme immunoassay for screening and quantitative analysis of erythromycin in various matrices.
Erythromycin is an antibiotic with a broad-spectrum activity, which belongs to the group of macrolides. Maximum Residue Limits (MRLs) for erythromycin for all food producing species are established in Europe: for tissue at 200 µg/kg (ppb), milk at 40 µg/kg and eggs at 150 µg/kg. The screening for tylosin and erythromycin is an important tool for control in the honey industry. The use of antibiotics in apiculture is forbidden. Therefore, no MRLs have been set for honey. Nevertheless, Recommended Concentrations (RCs) have been published by the European Union Reference Laboratories. For erythromycin the RC is 20 µg/kg.
The Erythromycin ELISA is a competitive enzyme immunoassay based on antibodies directed against erythromycin.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each).|
|Sample preparation||For the mentioned matrices, fast and efficient methods are included in the test kit description.|
|Incubation time||1 h 30 min|
|LOD (Detection limit)||Milk, urine 4 µg/L, liver, shrimp, egg and fish 10 µg/kg|
|Validated matrices||Milk, urine, liver, shrimp, egg and fish.|
|Detected analyte||Erythromycin in various matrices.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|