EuroProxima Florfenicol is a competitive enzyme immunoassay for the screening and quantitative detection of florfenicol in different commodities.
Florfenicol is an effective broad-spectrum antibiotic and an analogue of chloramphenicol. Due to the ban of the use of chloramphenicol in food-producing animals, florfenicol is used to control infections in humans, pigs, poultry, non-ruminating cattle and aquaculture. Within the EU, Maximum Residue
Limits (MRLs) for florfenicol have been established.
The EuroProxima Florfenicol ELISA is a competitive enzyme immunoassay based on monoclonal antibodies directed against florfenicol.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each).|
|Sample preparation||For mentioned matrices fast and efficient extraction methods are included in the kit description.|
|Incubation time||45 min (20-25 °C)|
|LOD (Detection limit)||Chicken/pork 0.2 µg/kg (ppb), fish/shrimp 0.2 µg/kg, egg 0.1 µg/kg|
|Detection capability CCβ||Chicken/pork 0.4 µg/kg (ppb), fish/shrimp 0.4 µg/kg, egg 0.4 µg/kg|
|Validated matrices||Chicken/pork, fish/shrimp and egg.|
|Detected analyte||Florfenicol in chicken/pork, fish/shrimp and egg.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|