RIDASCREEN® Nitrofuran (AHD) is a competitive enzyme immunoassay for the quantitative analysis of the nitrofurantoin metabolite AHD in shrimp and fish.
Nitrofurans are synthetic broad-spectrum antibiotics, which are frequently used in animal production due to their excellent antibacterial and pharmacokinetic properties. They have also been used as growth promoters during the production of shrimp, poultry and pigs. Long term animal experiments have shown that the parent compounds and their metabolites have carcinogenic and mutagenic characteristics. This led to the prohibition of nitrofurans for the treatment of animals used for food production. In 1993, the EU banned the nitrofurans furaltadone, nitrofurantoin and nitrofurazone for use in animals used as sources of food, and in 1995 the use of furazolidone was also prohibited.
The analysis of nitrofurans is based on the detection of the tissue bound metabolites of nitrofurans. The parent compounds are difficult to detect accurately since they are metabolized very rapidly after treatment. The tissue bound nitrofuran metabolites however are present for a long time after administration and they are used to detect nitrofuran abuse. Prior to analysis, the metabolites have to be derivatized by incubation with 2-Nitrobenzaldehyde (2NBA) into Nitrophenyl-(NP) AHD, NP-AMOZ, NP-AOZ and NP-SEM.
parent compound / metabolite (abbreviation) / after derivatizaton
Nitrofurantoin / 1-Aminohydantoin (AHD) / NP-AHD
Furaltadone / 3-Amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) / NP-AMOZ
Furazolidone / 3-Amino-2-oxazolidinone (AOZ) / NP-AOZ
Nitrofurazone / Semicarbazide (SEM) / NP-SEM
- One sample preparation for the nitrofurans AHD, AMOZ, AOZ and SEM as well as for chloramphenicol.
- High sensitivity – allows compliance with European legislation (MRPL value: 1000 ng/kg (ppt) – Commission Decision 2003/181/EC).
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each)|
|Sample preparation||Homogenization, derivatization, extraction, centrifugation, evaporation and defatting.|
|Incubation time||1 h 15 min|
|LOD (Detection limit)||Shrimp approx. 200 ng/kg, fish approx. 76 ng/kg|
|Validated matrices||Shrimp and fish.|
|Available application notes||Shrimp and fish.|
|Detected analyte||Nitrofuran (AHD) in shrimp and fish.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|