RIDASCREEN® Chloramphenicol is a competitive enzyme immunoassay for the quantitative analysis of chloramphenicol in milk, milk powder and dairy products, honey and royal jelly, meat, fish, shrimp, eggs, urine (also chloramphenicol glucuronide), plasma/serum and feed.
Chloramphenicol is a broad spectrum antibiotic, which is frequently employed in animal production for its excellent antibacterial and pharmacokinetic properties. However, in humans it leads to hematotoxic side effects, in particular chloramphenicol-induced aplastic anaemia for which a dosage-effect relationship has not yet been established. This has led to a prohibition of chloramphenicol for the treatment of animals used for food production. For test systems, a minimum required performance limit of 300 ng/kg (ppt) was established.
- Improved sensitivity and recovery.
- Less pipetting through functionalized microtiter plate.
- Only 45 minutes incubation time.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each)|
|Sample preparation||For the mentioned matrices fast and efficient methods are included in the test kit description, see under short information/ sample preparation.|
|Incubation time||45 min|
|LOD (Detection limit)||Milk approx. 24 ng/l, milk powder (reconstitution) approx. 240 ng/kg, milk powder (extraction) approx. 24 ng/kg, joghurt, kefir, buttermilk, cream approx. 12 ng/kg, curd, sour cream approx. 15 ng/kg, butter approx. 61 ng/kg, cheese approx. 16 ng/kg, honey approx. 25 ng/kg, royal jelly approx. 23 ng/kg, meat (beef, pork, poultry) approx. 5 ng/kg, fish approx. 8 ng/kg, shrimp approx. 8 ng/kg, shrimp (5 in 1 nitrofuran sample prep.) approx. 34 ng/kg, egg approx. 15 ng/kg, urine direct (CAP-glucuronide) approx. 138 ng/l, urine hydrolyzed (chloramphenicol) approx. 196 ng/l, plasma/ serum approx. 18 ng/l, feed approx. 107 ng/kg|
|Detection capability CCβ||Milk 75 ng/l, milk powder 100 ng/kg, yoghurt, kefir, buttermilk, cream 50 ng/kg, curd, sour cream 300 ng/kg, butter 150 ng/kg, cheese 200 ng/kg, honey (extraction) 50 ng/kg, royal jelly 100 ng/kg, meat 25 ng/kg, fish 25 ng/kg, shrimp 25 ng/kg, shrimp (nitrofuran sample preparation) 150 ng/kg, egg 75 ng/kg, urine (direct) 300 ng/kg, urine (after hydrolysis) 325 ng/kg, plasma 50 – 100 ng/kg, feed 150 ng/kg|
|Validated matrices||Milk, milk powder, yoghurt, kefir, buttermilk, cream, curd, sour cream, butter, cheese, honey, royal jelly, meat (beef, pork, poultry), fish, shrimp, eggs, urine, plasma/ serum, feed.|
|Available application notes||Shrimp|
|Detected analyte||Chloramphenicol in milk, milk powder and dairy products, honey and royal jelly, meat, fish, shrimp, eggs, urine (also chloramphenicol glucuronide), plasma/serum and feed.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|