EuroProxima Ivermectin is an unique competitive enzyme immunoassay (ELISA) for the detection of ivermectin in urine, tissue and milk samples.
Ivermectin, also named dihydroavermectin, is a member of a group of naturally occurring macrocyclic lactones known as avermectins. The following compounds belong to the avermectines: abamectin, ivermectin, doramectin and eprinomectin. Ivermectin is frequently used to control parasites in many food producing animal species. It is a mixture of two homologues containing not less than 80% 22,23-dihydroavermectin B1a and not more than 20% 22,23-dihydroavermectin B1b. It is an exceptionally potent drug which exhibits a broad spectrum activity against parasites in many species.
Within the European Union Maximum Residue Limits (MRL) for ivermectine have been established; based on the 22,23-dihydro-avermectin B1a marker residue.
The Ivermectin ELISA is a competitive enzyme immunoassay based on antibodies directed against ivermectin.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each).|
|Sample preparation||For the mentioned matrices fast and efficient methods are included in the kit description.|
|Incubation time||1 h 30 min (37 °C / 20 – 25 °C)|
|LOD (Detection limit)||Milk 5 µg/l (ppb), corned beef 5 µg/kg, liver 8 µg/kg, serum 1 µg/l, urine 4 µg/l, tissue 3 µg/kg|
|Detection capability CCβ||Corned beef 5 µg/kg, liver 12.5 µg/kg, tissue 6 µg/kg|
|Validated matrices||Milk, corned beef, liver, serum, urine and tissue.|
|Detected analyte||Ivermectin in milk, corned beef, liver, serum, urine and tissue.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|