RIDASCREEN® Aflatoxin B1 30/15 is a competitive enzyme immunoassay for the quantitative determination of aflatoxin B1 in cereals and feed.
Aflatoxins are secondary metabolites of the fungi species Aspergillus flavus, parasiticus and nomius. These fungi occur in humid tropical areas and the contamination of vegetable food takes place in the cultivable countries. Aflatoxins belong to the strongest natural occurring carcinogenic substances. Aflatoxin B1 appears nearly in all cases together with Aflatoxin B2 , G1 and G2 and it is the analyte with the highest toxic significance. It is commonly found in corn, peanuts, brazil nuts, cotton seed and pistachios. Due to the toxicity of these mycotoxins maximum levels for Aflatoxin B1 and total aflatoxins for food and feed apply in EU countries.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each)|
|Sample preparation||Grinding, extraction, filtration or centrifugation and dilution.|
|Incubation time||45 min|
|LOD (Detection limit)||Cereals 1 µg/kg (ppb), soy 1.7 µg/kg, feed 4 µg/kg, dry cat food 2 µg/kg|
|Validated matrices||Cereals, soy, feed and dry cat food.|
|Detection range||1 – 50 µg/kg|
|Detected analyte||Aflatoxin B1 in cereals and feed.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|