RIDASCREEN® Aflatoxin Total is a competitive enzyme immunoassay for the quantitative determination of aflatoxins in cereals and feed.
Aflatoxins are secondary metabolites of the fungi species Aspergillus flavus, parasiticus und nomius. These fungi occur in humid tropical areas and the contamination of vegetable food takes place in the cultivable countries. Aflatoxins belong to the strongest natural occurring cancerogenic substances. Aflatoxin B1 appears nearly in all cases together with Aflatoxin B2 , G1 and G2 and it is the analyte with the highest toxic significance. It is found above all in corn, peanuts, brazil nuts, cotton seed and pistachios. Due to the toxicity of these mycotoxins maximum levels for Aflatoxin B1 and total Aflatoxins for food and feed apply in EU countries.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each)|
|Sample preparation||Grinding, extraction, filtration or centrifugation and dilution.|
|Incubation time||45 min|
|LOD (Detection limit)||1.75 µg/kg (ppb) (cereals and feed)|
|Validated matrices||Cereals and feed.|
|Available application notes||An application note for green coffee in combination with RIDA® Aflatoxin column (Art. No. R5001/R5002) is available on request.|
|Detection range||1.75 – 141.75 µg/kg|
|Detected analyte||Aflatoxin in cereals and feed.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|