RIDASCREEN® DON is a competitive enzyme immunoassay for the quantitative analysis of DON in cereals, malt, feed, beer and wort.
Deoxynivalenol belongs to the trichothecene group of mycotoxins and is formed by fungi of the genus Fusarium. Deoxynivalenol often occurs in plant products particularly in cereals. More as 150 trichothecenes are known and the mycotoxins deoxynivalenol, 3-acetyl- and 15-acetyl-deoxynivalenol are the toxins most frequently occurring in Europe and Northern America. The toxin concentrations found in wheat, corn or rice are often in the µg/kg (ppb) range. Due to their high cytotoxic and immunosuppressive properties these toxins pose a risk to human and animal health.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each)|
|Sample preparation||Cereals, malt, feed: extraction, filtration. Beer: removing of CO2. Wort: no sample preparation necessary.|
|Incubation time||45 min|
|LOD (Detection limit)||Cereals, malt, feed approx. 18.5 µg/kg, beer 3.7 µg/l, wort 3.7 µg/l|
|Validated matrices||Cereals, malt, feed, beer and wort.|
|Detection range||Cereals, malt, feed 18.5 – 500 µg/kg, beer 3.7 – 100 µg/l, wort 3.7 – 100 µg/l|
|Detected analyte||DON in cereals, malt, feed, beer and wort.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|