RIDASCREEN®FAST Almond is a sandwich enzyme immunoassay for the quantitative analysis of almond or parts of almond in food. As representative samples for the food groups pastries and sweets the following matrices have been tested during the assay´s development: cereals, cookies, ice cream and chocolate. The assay can be likely used for analysis of other food samples too; this has to be checked by the user.
The allergen can be present as an ingredient or as a contamination in raw and cooked products. According to the regulation (EU) No. 1169/2011, almond must be declared on food labels as it can induce allergic reactions. Similar regulations exist e.g. in the USA, Canada, Australia and New Zealand.
- Widespread testing for cross reactivity (80 matrices have been evaluated).
- Accuracy: recovery of spiked samples was between 66 – 124 % (mean: 97.8 %).
- Validated for automates.
|Test format||Microtiter plate with 48 wells (6 strips with 8 removable wells each)|
|Sample preparation||Homogenization, extraction and centrifugation|
|Incubation time||30 min
|LOD (Detection limit)||0.1 mg / kg (ppm) almond (mean)
0.0 – 0.23 mg / kg (ppm) almond*
*depending on matrix
|LOQ (Limit of quantification)||2.5 – 20 mg / kg (ppm) almond
(depending on matrix, level of food processing and extraction method)
|Cross reactivity||Cross reaction to apricot stone, mulberries, mahaleb cherry (cross reactions to other plants from genus Prunus are possible).|
|Validated matrices||Representative samples for the food groups pastries and sweets: cereals, cookies, ice cream and chocolate.|
|Available application notes||
|Detected analyte||Proteins from almond|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|