The SureFood® ALLERGEN 4plex Peanut/Hazelnut/Walnut+IAC is a real-time PCR for the direct, qualitative detection and differentiation of specific Peanut (Arachis hypogaea), Hazelnut (Corylus maxima) and Walnut (Juglans regia, Juglans nigra) DNA sequences according to directive (EC) 1169/2011.
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Applied Biosystems 7500, Bio-Rad CFX96, R-Biopharm RIDA®CYCLER, Roche LightCycler® 480 II and Qiagen Rotor-Gene Q.
- SureFood® PREP Advanced
- SureFood® QUANTARD Allergen 40
- SureFast® Mag PREP Food
- TANBead Maelstrom™ 8 Autostage
|Test format||100 reactions|
|Sample preparation||For DNA preparation, the use of SureFood® PREP Advanced (S1053), protocol 1, is recommended.|
|LOD (Detection limit)||≤ 1 mg / kg peanut
≤ 0.4 mg / kg hazelnut
≤ 0.4 mg / kg walnut
|Evaluation||Real-time PCR instrument with four detection channels (510 nm, 580 nm, 610 nm and 660 nm)|