Description
Intended use:
The real-time PCR test detects DNA of celery (Apium graveolens) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC).
General Information:
For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 (S3301) containing 40 mg celery / kg food sample and a detection system including a standard dilution is required.
For DNA preparation, the use of SureFood® PREP Advanced (S1053), protocol 1, is recommended.
The real-time PCR assay can be used with established real-time PCR instruments capable of detection of at least two fluorescence dyes. Internal validation was performed on Roche LightCycler® 2.0, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, R-Biopharm RIDA®CYCLER, LTF MyGo Pro, AriaDx and Agilent.
The method is validated for quantitative determination of 1 mg to 400 mg allergenic substance / kg food sample using the reference material SureFood® QUANTARD Allergen 40 containing 40 mg allergenic substance / kg food sample.
Accessories:
| Article Numbers | S3605 |
|---|---|
| Test format | 100 reactions |
| LOD (Detection limit) | ≤ 0.4 mg / kg |
| LOQ (Limit of quantification) | 1 mg / kg using SureFood® PREP Advanced, protocol 1 |
| Evaluation | Real-time PCR instrument with two detection channels (510 nm and 580 nm) |
| Instructions | |
|---|---|
| MSDS |
