SureFood® ALLERGEN Crustaceans

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The SureFood® ALLERGEN Crustaceans real-time PCR test detects DNA of crustaceans (Crustacea) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC).

 

Description

Intended use:

The SureFood® ALLERGEN Crustaceans real-time PCR test detects DNA of crustaceans (Crustacea) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC).

 

General Information:

For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 (S3301) containing 40 mg crustaceans / kg food sample and a detection system including a standard series is required.

For DNA preparation, the use of SureFood® PREP Advanced (S1053), protocol 1, is recommended.

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC, Agilent AriaDx and Agilent Mx3005P.

The method is validated for quantitative determination of 1 mg to 400 mg allergenic substance / kg food sample using the reference material SureFood® QUANTARD Allergen 40 containing 40 mg allergenic substance / kg food sample.

 

Accessories:

 

Specifications
Article Numbers S3612
Test format 100 reactions
LOD (Detection limit) ≤ 0.4 mg / kg
LOQ (Limit of quantification) 1 mg / kg using SureFood® PREP Advanced, protocol 1
Cross reactivity Cross reactivity was observed with DNA extracts from abalone (Haliotis spp.) with 100 %.
Evaluation Real-time PCR instrument with two detection channels (510 nm and 580 nm)
Files
Instructions
MSDS

Additional information

Allergens analyis