EuroProxima Oxolinic acid is a competitive enzyme immunoassay for the screening and quantitative detection of oxolinic acid in fish, shrimp and prawn.
Oxolinic acid is a synthetic antimicrobial agent belonging to the first quinolone class of antibiotics. Oxolinic acid is used in veterinary medicine for treatment of cattle, pigs, poultry, fish and shrimp. After antibiotic treatment, the level of residues in animal derived products should be low to limit human exposure to these drug residues. In the European Union in accordance with Commission Regulation (EU) No 37/2010 the maximum residue limits (MRLs) for oxolinic acid in animal derived products are 100 μg/kg (ppb) in muscle, 50 μg/kg in fat, 150 μg/kg in liver and 150 μg/kg in kidney.
The EuroProxima Oxolinic acid ELISA is a competitive enzyme immunoassay based on polyclonal antibodies directed against oxolinic acid.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each).|
|Sample preparation||For mentioned matrices fast and efficient extraction methods
are included in the kit description.
|Incubation time||1 h (20 – 25 °C)|
|LOD (Detection limit)||Fish 2 µg/kg, prawn/shrimp 2 µg/kg|
|Detection capability CCβ||Fish 3 µg/kg, prawn/shrimp 3 µg/kg|
|Validated matrices||Fish and prawn/shrimp.|
|Detected analyte||Oxolinic acid in fish, and prawn/shrimp.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|