RIDASCREEN® Nitrofuran (AOZ) is a competitive enzyme immunoassay for the quantitative analysis of AOZ in shrimp, meat (chicken, pork, beef), liver (beef and pork), fish, whole egg and milk.
Nitrofurans are synthetic broad-spectrum antibiotics, which are frequently used in animal production due to their excellent antibacterial and pharmacokinetic properties. They have also been used as growth promoters during the production of shrimp, poultry and pigs. Long term animal experiments have shown that the parent compounds and their metabolites have carcinogenic and mutagenic characteristics. This led to the prohibition of nitrofurans for the treatment of animals used for food production. In 1993, the EU banned the nitrofurans furaltadone, nitrofurantoin and nitrofurazone for use in animals used as sources of food, and in 1995 the use of furazolidone was also prohibited.
The analysis of nitrofurans are based on the detection of the tissue bound metabolites of nitrofurans. The parent compounds are difficult to detect accurately since they are metabolized very rapidly after treatment. The tissue bound nitrofuran metabolites however are present for a long time after administration and they are used to detect nitrofuran abuse.
Prior to analysis, the metabolites have to be derivatized by incubation with 2-Nitrobenzaldehyde into NP-AHD, NP-AMOZ, NP-AOZ and NP-SEM.
- Sensitive: The detection limit is approx. 50 ng/kg (ppt) in shrimp, fish and milk. The detection limit is approx. 100 ng/kg in meat, liver and whole egg.
- Rapid: Assay results within 1 h 15 minutes.
- Simple: Test can be performed immediately.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each)|
|Sample preparation||Homogenization (milk samples: precipitation), derivatization, extraction, centrifugation, evaporation and degreasing.|
|Incubation time||1 h 15 min|
|LOD (Detection limit)||Shrimp, fish, milk approx. 50 ng/kg, meat, liver, whole egg approx. 100 ng/kg|
|Validated matrices||Shrimp, meat (chicken, pork, beef), liver (beef and pork), fish, whole egg and milk.|
|Available application notes||Fish and shrimp.|
|Detected analyte||Nitrofuran (AOZ) in shrimp, meat (chicken, pork, beef), liver (beef and pork), fish, whole egg and milk.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|